Becton Dickinson and Company Limited

Axel A Yup

Song Shi

John D Mantlo

Xiao Li

Kenneth Anthony Kopher

William Alfred Pope

Yongqiang Zhang

Described herein are methods and reagents for identifying and analyzing at least one microorganism (e.g. bacteria) in a sample and reducing the background signal intensity obtained when analyzing the sample by flow cytometry. The sample is prepared by combining the sample with a background signal-reducing molecule or with a nucleic acid stain covalently linked to a quencher. A portion of the particulate matter in the sample can optionally be removed with a resin prior to staining with a nucleic acid stain.

Sample preparation for flow cytometry

A process for determining one of the presence, absence, or total of microorganisms (e.g. bacteria) in a sample. According to the process, a biological sample containing complex matrices is obtained. The sample is first combined with a resin to adsorb complex matrices from the sample. The resin is removed from the biological sample. The sample so prepared is then analyzed by flow cytometry.

Method for improving analysis of microorganisms in complex matrices

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Owings Mills, MD, United States of America

Axel A Yup