Pacific Biosciences of California, Inc.

Applied Biosystems, LLC

Applera Corporation

Austin B Tomaney

Mark F Oldham

Patrick Marks

David J Holden

Kenneth Mark Maxham

Devon Murphy

Kevin Hester

Stephen Wilson Turner

Jonas Korlach

Jeremiah Hanes

Kevin Travers

Cheryl Heiner

Jon M Sorenson

Benjamin A Flusberg

Dale R Webster

Andrey Kislyuk

John Lyle

Jeffrey Wegener

Lei Jia

Tyson A Clark

Igor D Vilfan

Jessica Lee

Joseph Puglisi

Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

Classification of nucleic acid templates

A system and method for characterizing contributions to signal noise associated with charge-coupled devices adapted for use in biological analysis. Dark current contribution, readout offset contribution, photo response non-uniformity, and spurious charge contribution can be determined by the methods of the present teachings and used for signal correction by systems of the present teachings.

Signal noise reduction for imaging in biological analysis

Identification of nucleic acid template-linked barcodes comprising nucleic acid modifications

Computer implemented methods, and systems performing such methods for processing signal data from analytical operations and systems, and particularly in processing signal data from sequence-by-incorporation processes to identify nucleotide sequences of template nucleic acids and larger nucleic acid molecules, e.g., genomes or fragments thereof.

Methods and processes for calling bases in sequence by incorporation methods

Methods for identifying nucleic acid modifications

Methods and apparatus for improving the resolution of an electronic imaging device having an array of pixels. Sub-pixel dimension movements between an object and the array of pixels are made, and an image is formed at each position. Resulting shifted images are combined to yield an effective resolution corresponding to an array having smaller pixels. Such methods and apparatus allow optical systems with existing pixel devices to form effective images of smaller feature sizes.

Image enhancement by sub-pixel imaging

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San Francisco, CA, United States of America

Austin B Tomaney