Roquette Freres

Aurore Satre-Buisson

Samuel Patinier

Michel Perrut

Philippe Looten

Eric Francais

The invention relates to a method for preparing a composition rich in lutein produced by microalgae, particularly microalgae belonging to thegenus, more specifically produced by, characterized in that said method includes the sequence of the following steps: 1) preparing a cell lysate from the microalgae biomass; 2) treating the lysed microalgae biomass with a polar solvent, preferably ethyl acetate, in order to obtain an oleoresin containing the lutein and the lipids from the initial biomass; 3) extracting the oleoresin obtained in step 2) by means of a non-polar solvent, here a fluid at supercritical pressure, in order to obtain a fraction rich in non-polar lipids, mainly consisting of triglycerides, and an insoluble fraction rich in lutein; and 4) recovering the thus lutein-enriched fraction.

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Champigneulles, France

Aurore Satre-Buisson