10X Genomics, Inc.

Augusto Manuel Tentori

Benjamin J Hindson

Michael Schnall-Levin

Hanyoup Kim

Rajiv Bharadwaj

David Sukovich

Layla Katiraee

Felice Alessio Bava

Pratomo Alimsijah

Siyuan Xing

Bill Kengli Lin

Ashkan Beyranvand Nejad

Spontaneous Rose McKnight Russell

Seayar Mohabbat

Janine Hensel

Lauren Gutgesell

Denis Pristinski

Cedric Uytingco

David Maurice Cox

Christopher Wing

Nabil Mikhaiel

Andrew D Price

Michael Ybarra Lucero

Tarjei Sigurd Mikkelsen

Tobias Daniel Wheeler

Colin Bruce Kennedy

Hendricus Marindra

Frances Yun

Joakim Lundeberg

Zachary Bent

Yifeng Yin

Steven W Short

Eswar Prasad Ramachandran Iyer

Caroline Julie Gallant

Neil Ira Weisenfeld

Mohammad Rahimi Lenji

Jennifer Chew

David Michael Patterson

Jerald Sapida

Dongyao Li

Shea Thompson Lance

Linda Kvastad

Martin Sauzade

Lynna Chen

Daniel Freitas

Fernandino Valdecanas

Reynaldo Farias Zorrilla

Meghan L F Frey

William Nitsch

Sultan Doganay Tuncer

Francis Cui

Erica Graziosa

Amanda Janesick

Eva Gracia Villacampa

Spontaneous Rose Mcknight Russell

Sample handling device

Provided herein is a fluid delivery method for permeabilizing a biological sample. The method includes delivering the fluid to a first substrate and/or a second substrate. At least one of the first substrate and the second substrate includes a spacer. The method further includes assembling, subsequent to the delivering, a chamber comprising the first substrate, the second substrate, the biological sample, and the spacer. The spacer may be disposed between the first substrate and second substrate. The spacer may be configured to maintain the fluid within the chamber and maintain a separation distance between the first substrate and the second substrate. The spacer may be positioned to at least partially surround an area on the first substrate on which the biological sample is disposed and/or at least partially surround the array disposed on the second substrate.

Fluid delivery methods

Display screen or portion thereof with transitional graphical user interface

Provided herein are methods of determining efficiencies of spatial transcriptomics methods.

Materials and methods for spatial transcriptomics

Various implementations of electrophoretic systems and instruments are provided to improve electrophoresis and ease of use. Various electrophoretic systems and instruments utilize different electrode designs that can result in uniform electromigration of analytes. The electrophoretic systems and instrumentations optimize the size and/or location of electrodes relative to a sample, thereby increasing uniformity of electric field and reducing or minimizing drift of analyte migration. In addition, the electrophoresis systems and instruments provide a solution to conveniently check for electrical connections in the systems and instruments, and alert users of potential improper electrical connections, prior to performing electrophoresis.

Electrophoresis cassettes and instrumentation

A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.

Methods of capturing multiple analytes on a spatial array

Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.

Methods, compositions, and systems for spatial analysis of analytes in a biological sample

Provided herein are materials and methods for in situ labeling of a biological sample for use in spatial analysis of one or more analytes in the biological sample. In some aspects, in situ labeling of a biological sample includes labeling the biological sample with one or more spatial barcodes.

In situ spatial barcoding

A method for aligning a sample to an array is provided. An image of sample image of a sample can be received by a data processor. The sample image having a first resolution. An array image including an overlay of an array with the sample and an array fiducial can be received by the data processor. The array image having a second resolution lower than the first resolution of the sample image. The sample image can be registered to the array image by aligning the sample image and the array image. An aligned image can be generated based on the registering. The aligned image can include an overlay of the sample image with the array. The aligned image can be provided by the data processor. A method for detecting fiducials associated with an array is provided. Systems and non-transitory computer readable mediums performing the method are also provided.

Sample handling apparatus and image registration methods

Methods, compositions, and systems for capturing probes and/or barcodes

The present disclosure provides electrophoretic systems, methods and compositions for spatial analysis, which can serve to magnify or demagnify spatial resolution of analytes of interest that are captured using electrophoresis. Some implementations can use a diverging or converging electric field in an electrophoretic capture system. Such a divergent or convergent electric field, as opposed to a parallel electric field, can be generated by, for example, utilizing different sizes of electrodes associated with or imbedded in substrates. Also provided herein are electrophoretic systems, methods and compositions for spatial analysis, which can serve to selectively migrate one or more analytes from a region of interest in the biological sample for capture using electrophoresis.

Electrophoretic methods for spatial analysis

A method of self-testing an imaging system of a sample handling apparatus is provided. Systems and non-transitory computer readable mediums performing the method are also provided.

Self-test for imaging device

Provided herein are methods, compositions, and systems for improved in situ detection of analytes and spatial analysis using, e.g., a sequencing readout.

Methods for improved in situ detection of nucleic acids and spatial analysis

Imaging system hardware

Growing community of inventors

Pleasanton, CA, United States of America

Augusto Manuel Tentori