Consortium Fur Elektrochemische Industrie Gmbh

Boehringer Mannheim Gmbh

August Bock

Anton Candussio

Gerhard Schmid

Michael Jarsch

Frank Muller

Angelika Birkmann

Konrad Lechner

Otto Huber

Florian Binder

Verena Schlensog

Dagmar Mayer

Roland Herbst

Robert Gary Sawers

Otto S Huber

An expression system can be regulated by acetate, pH and oxygen, which expression system includes a trans-acting regulator protein and a promoter which can be activated by this protein. Any desired structural genes are maximally expressed under the control of the expression system at an oxygen partial pressure, pO.sub.2, of 0-5% and a pH of 6.0-6.5, and in the presence of acetate at a concentration of 40-60 mM. There is also a process for preparing this expression system, and a process for using this expression system.

Expression system which can be regulated

The recombinant DNA according to the present invention contains a regulator region which is different from the pfl gene; a promoter region upstream from the gene which contains a -35/-10 promoter sequence; a regulator region upstream from the promoter region which contains a sequence (I) 5'-GAGATATGATCTATATCAATTTC-3' or a 23 base pair sequence which is at least 80% identical to positions 6-10 and 15-19 of said sequence (I) but not having C at position 8 and/or G at position 17. An expression vector according to the present invention contains a recombinant DNA according to the present invention ligated into a suitable vector molecule and methods are also described for expression the DNA.

Recombinant DNA and expression vector for the repressible and inducible

The invention relates to DNA fragments having the cyclodextrin glycosyltransferase structural gene, to vectors and microorganisms for expression and to a preparation process for the same.

DNA-fragment having the cyclodextrin glycosyltranferase gene

The alkalophilic bacteria (DSM 5853) possesses a maltopentaose producing amylase. The maltopentaose producing amylase, and its derivatives modified by gene manipulation, can be expressed in E. coli. These amylases facilitate the production of maltopentaose.

Biologically pure culture of maltopentaose forming amylase producing

A maltopentaose producing amylase, and its derivatives modified by gene manipulation, can be expressed in E. col. These amylases facilitate the production of maltopentaose.

Maltopentaose producing amylases

The invention relates to Aureobasidium pullulans strains which produce pullulan but virtually no melanin. A process for the preparation of these strains, and a preferred use, are also disclosed.

Aureobasidium pullulans strain, process for its preparation, and use

The present invention provides a recombinant DNA, wherein it contains the consensus sequence: ##STR1## and a promoter which has the DNA sequence GGN.sub.10 GC. The present invention also provides a process for the isolation of this recombinant DNA, wherein a DNA sequence containing the consensus sequence is identified from a gene bank of a micro-organism, which contains a gene which is repressible by oxygen and inducible by formate under anaerobic conditions, isolated and combined with an appropriate promotor according to known methods. This recombinant gene can be used for the inducible and repressible expression of a foreign gene by bringing about the induction by formate under anaerobic conditions and the repression by oxygen.

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Geltendorf, Germany

August Bock