Solvo Biotechnológial Zrt.

Eleszto Genetika, Inc.

Solvo Biotechnology

Attila Németh

Hristos Glavinas

Lajos Szente

Balázs Sarkadi

Abhinav Kumar

András Váradi

Ágnes Telbisz

Lorand Szabo

György Báthori

Szilvia Varszegi

Dóra Méhn

Szilvia Gedey

Bela Krisztian Szamecz

Éva Fenyvesi

Emese Kis

Pál Ákos

Tünde Nagy

Péter Krajcsi

Éva Molnár

Ákos Pál

Disclosed herein are microorganism and methods that can be used for the synthesis of cannabigerolic acid (CBGA) and cannabinoids. The methods disclosed can be used to produce CBGA, Δ-tetrahydrocannabinolic acid (THCA), cannabidiolic acid (CBDA), cannabichromenic acid (CBCA), Δ-tetrahydrocannabinol (THC), cannabidiol (CBD), cannabichromene (CBC). Enzymes useful for the synthesis of CBGA and cannabinoids, include but are not limited to acyl activating enzyme (AAE1), polyketide synthase (PKS), olivetolic acid cyclase (OAC), prenyltransferase (PT), THCA synthase (THCAS), CBDA synthase (CBDAS), CBCA synthase (CBCAS), HMG-Co reductase (HMG1), and/or farnesyl pyrophosphate synthetase (ERG20). The microorganisms can also have one or more genes disrupted, such as gene that that controls beta oxidation of long chain fatty acids.

Microorganisms and methods for the fermentation of cannabinoids

The invention provides for a novel cholesterol loaded insect cell membrane preparation having an increased cholesterol level as compared to physiological cholesterol levels of insect cell membranes or to control insect cell membrane preparations without cholesterol loading, wherein said cholesterol loaded membrane preparation comprises an ABC transporter protein having an increased substrate transport activity due to increased cholesterol level of the membrane. The invention also relates to reagent kits comprising the preparations of the invention. The invention also relates to methods for manufacturing said preparations and methods for measuring any type of activity of the ABC transporters present in the cholesterol loaded membranes as well as studying or testing compounds and interaction of compounds and ABC transporters, in this assay systems. The invention also provides for a test system useful for testing whether ABC transporter proteins can be activated by cholesterol in an insect cell membrane.

Cholesterol loaded insect cell membranes as test proteins

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Budapest, Hungary

Attila Németh