Digene Corporation

Attila L&ouml;rincz

Ming Chan

Sharon Challberg

Mariana Garcia

Chaka Impraim

Allison Cullen

A non-radioactive hybridization assay and kit for the detection of genetic defects, microbial infections or viral infections, such as human papillomavirus. A test sample is treated with a base and incubated with nucleic acid probes, diluted in a neutralizing buffer, specific for target nucleic acids. The hybrids are captured onto a solid phase coated with an anti-hybrid antibody, unhybridized probe is eliminated, and the bound hybrid detected.

Non-radioactive hybridization assay and kit

An improved assay disclosed for detecting viral nucleic acid sequences. The improvement involves concentration viral particles from a biological sample by centrifugation. Once concentrated, the nucleic acid molecules in the viral particles can be manipulated or detected using any suitable procedure. Many such procedure are know and can be used with the concentrated viral nucleic acid molecules. Preferably, the concentrated viral nucleic acid is detected using a detection assay.

Growing community of inventors

Gaithersburg, MD, United States of America

Attila Lörincz

Growing community of inventors

Gaithersburg, MD, United States of America

Attila L&ouml;rincz

Attila Lörincz