Codexis, Inc.

Institute for Drug Research Ltd.

Other

Biogal Gyogyszergyar Rt.

Biogal Gyogyszergyar

Attila Andor

Xiyun Zhang

Jie Yang

Gabor Ambrus

Istvan M Szabo

Karoly Albrecht

Eva Ilkoy

Antonia Jekkel

Imre Moravcsik

Louis Clark

Lisa Marie Newman

Dipnath Baidyaroy

Gyula Horvath

Sally Rhiannon Postlethwaite

Istvan Barta

Gyorgy Somogyi

Ildiko Lang

Julianna Mozes Nee Suto

Margit Bidlo Nee Igloy

Sandor Boros

Attila Konya

Janso Salat

Janos Erdei

Gyorgy Santha

Kalman Polya

Laszlo Birincsik

Lajos Kiss

Karoly Nagy

Valeria Szell

Laszlo Cseke

Antonia Jekkel Nee Bokany

Kalman Konczol

Eva Toth-Sarudy

Bela Makadi

Mihaly Kaszas

Eva Tomori Nee Joszt

Ilona Varga

Istvan Magyi

Ilona Bagdi

Edit Halasz

Attila Jakab

Lajos De&aacute;k

J&aacute;nos B&aacute;lint

Istv&aacute;n Szab&oacute;

Laszlo Birinesik

Zsuzsanna Scheidl

Etelka Deli

Csaba Szab&oacute;

Gyula Horv&aacute;th

Istvan Mihaly

Ildik&oacute; L&aacute;ng

Agota Hulber Nee Dobos

Imre Sz&eacute;kely

Vilmos K&eacute;ri

Vera Kov&aacute;cs

Bela Palotas

Zsuzsanna Szt&aacute;ray

Karoly Buzasi

Andras Kiss

Ant&oacute;nia Jekkel

Etelka Deli Nee Konszky

Irma H&ouml;gye

Szabolcs M&aacute;ty&aacute;s

Aniko Molnar Nee Antal

L&aacute;szl&oacute; Eszenyi

Vilma Szaszhegyesi

G&aacute;bor Ambrus

&Eacute;va Ilk&ouml;y

Janos Salat

The invention relates to recombinantly produced &#x3b2;-glucosidase variants with enhanced thermoactivity compared to naturally occurring proteins. The invention also provides methods for producing a variant &#x3b2;-glucosidase polypeptide with improved thermoactivity by identifying performance sensitive positions in a target &#x3b2;-glucosidase polypeptide and substituting the residue at that position with a thermoactivity enhancing residue.

Beta-glucosidase variants

&#x3b2;-glucosidase variants

The invention relates to recombinantly produced &#x3b2;-Glucosidase Variants with enhanced thermoactivity compared to naturally occurring proteins. The invention also provides methods for producing a variant &#x3b2;-glucosidase polypeptide with improved thermoactivity by identifying performance sensitive positions in a target &#x3b2;-glucosidase polypeptide and substituting the residue at that position with a thermoactivity enhancing residue.

&#x3b2;-Glucosidase variants

The invention relates to recombinant expression of a variant form of a fungal C1 strain &#x3b2;-glucosidase. The invention also relates to the generation of fermentable sugars from biomass and the production of biofuels by fermentation of the sugars using genetically modified organisms expressing the &#x3b2;-glucosidase variant. The invention provides methods for producing a fermentable sugar, such as glucose, from cellobiose by contacting cellobiose with a recombinant &#x3b2;-glucosidase variant protein, such as a variant protein secreted by a recombinant host cell into culture medium. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.

Recombinant &#x3b2;-glucosidase variants for production of soluble sugars from cellulosic biomass

In a process for preparing mevinolin by fermentation of a biomass in a fermentation liquor, which includes dissolving mevinolin from the biomass into the fermentation liquor, and separating the biomass from the fermentation liquor to obtain a separated fermentation liquor, separating the mevinolin from the separated fermentation liquor, and recovering the end product, the improvement which comprises carrying out the dissolving at a pH between 7.5 and about 10, and the separating of the mevinolin is carried out at a pH between about 4.5 and about 1.

Process for the isolation and purification of mevinolin

Microbial process for preparing pravastatin

The invention refers to a new microbial process for preparing .beta.,.delta.-dihydroxy-7-[1,2,6,7,8,8a-hexahydro-2.6-dimethyl-8-(2-methy l-butyryloxy)-naphthalen-1-yl]-heptanoic acid .delta.-lactone of formula (I) and .beta.,.delta.-dihydroxy-7-[1,2,6,7,8,8a-hexahydro-2.6-dimethyl-8-(2-methy lbutyryloxy)-naphtalen-1-yl]-heptanoic acid of formula (II), ##STR1## by the aerobic fermentation of the submerged culture of an imperfect fungus strain biosynthesizing the above compounds, in a nutrient medium containing utilizable carbon and nitrogen sources as well as mineral salts, and by isolating the product of formula (I), which comprises cultivating a strain of the new Aspergillus obscurus fungus species producing the above compound(s) of formulas (I) and/or (II), in a temperature range of 25.degree. to 30.degree. C. and, if desired, separating the product(s) formed from the fermentation broth, then isolating it in the lactone form of formula (I) and, if desired, purifying the same.

Microbial process for preparing mevinolin by a strain of aspergillus

The invention relates to a microbial process for the production of immunosuppressive cyclosporine antibiotic complex or of its components, cyclosporine A, cyclosporine B and cyclosporine C, by the aerobic fermentation of a filamentous fungus strain biosynthesizing the above antibiotic(s) in a nutrient medium containing utilizable carbon and nitrogen sources as well as mineral salts, and by isolating the products formed, which comprises culturing a strain of the novel Tolypocladium varium fungus species producing the cyclosporine antibiotic complex, preferably tolypocladium varium sp. nov. CY/93, deposited at the National Collection of Agricultural and Industrial Microorganisms, Budapest, Hungary under the number NCAIM(P)F 001005, on a nutrient medium containing carbon sources, organic and inorganic nitrogen sources as well as mineral salts, under aerobic conditions, at 25.degree. to 30.degree. C., and, if desired, isolating and purifying the cyclosporine antibiotic complex or its components produced.

Microbial process for the production of immunosuppressive antibiotics

Growing community of inventors

Budapest, Hungary

Attila Andor