Toyo Boseki Kabushiki Kaisha

National Institute of Advanced Industrial Science and Technology

Toyobo Co., Ltd.

Atsushi Sogabe

Yoshihisa Kawamura

Yoshiaki Nishiya

Shigenori Emi

Shuhei Yamamoto

Kazumi Yamamoto

Masanori Oka

Seiji Takeshima

Yukihiro Sogabe

Tomotake Morita

Dai Kitamoto

Takashi Hattori

Takahide Kishimoto

Shinichi Teshima

Shizuo Hattori

Masaru Kitagawa

Azusa Saika

Tokuma Fukuoka

Michiko Suzuki

Tomohiro Sugahara

Yuzuru Suzuki

Yukio Takii

Takeshi Tanaka

Yutaka Takarada

Atsunori Hiratsuka

Kenji Inagaki

Toshihiro Kuroita

Keizo Yoneda

Hitoshi Muguruma

Takashi Hatta

Shigeo Shibatani

Makoto Ashiuchi

Tomohiro Imura

Hisanori Iwasa

Hiroaki Kitazawa

Michiyo Minami

Naoki Tanaka

Hiroshi Nishise

Hideaki Koike

Katsumi Tsuji

Kouhei Orihara

Provided is a method for efficiently producing a monoacyl MEL. The method comprises culturing a monoacyl-MEL-producing microorganism in the presence of a surfactant.

Production of monoacyl MEL

Provided is a means for increasing mannosylerythritol lipid (MEL) production efficiency. The present invention is a mannosylerythritol-lipid-producing microorganism transformed with an expression vector having a gene that encodes a lipase under the control of E5Pgap promoter or E5Ptef promoter.

Biosurfactant-producing recombinant microorganism

Provided is a means for promoting electron transfer between nanocarbon and other substances. An electron transfer accelerator for nanocarbon comprising a compound having an aromatic ring Skelton.

Electron transfer by nanocarbon

There are provided a microorganism characterized by producing poly-&#x3b3;-L-glutamate with a molecular weight of 1,300,000 or greater and uniform optical purity under liquid culture conditions, a method of screening for the microorganism, a method of producing poly-&#x3b3;-L-glutamate having large molecular weight by using the microorganism, and poly-&#x3b3;-L-glutamate having an average molecular weight of 1,300,000 or greater. In addition, usages of these are provided.

&#x3b3;-L-PGA producing microorganism, method of producing &#x3b3;-L-PGA using the microorganism, crosslinked substance produced using the microorganism, and external dermal agent produced using the microorganism

It is intended to provide by improving a known method of producing hyaluronic acid in a plant, a plant or a cultured plant cells which can produce hyaluronic acid at a lower cost and a further higher yield than before, a method of preparing the same, an expression vector for transformation, a method of producing hyaluronic acid using the plant or the cultured plant cells and the like. The method of producing hyaluronic acid comprising obtaining hyaluronic acid by co-expressing a protein with hyaluronic acid synthase activity and an exogenous protein with sugar-nucleotide synthase activity in a plant cell or a plant is provided.

Plant producing hyaluronic acid

The present invention includes as an active ingredient at least one biosurfactant, in particular mannosyl alditol lipid (such as MEL and MML) or triacylated mannosyl alditol lipid. This allows providing an activator and anti-aging agent that is excellent in activating and anti-aging effects on cells and that is safe enough to be used for a long time, and also providing cosmetics, quasi-drugs, drugs, and drinks and foods including the activator and the anti-aging agent as active ingredients. Further, the present invention provides MEL whose mannosyl erythritol skeleton in a molecular structure is 1-O-&#x3b2;-D-mannopyranosyl-meso-erythritol and a method for producing the MEL with use of a microorganism.

Activator including biosurfactant as active ingredient, mannosyl erythritol lipid, and production method thereof

The disclosure relates to (i) a gene isolated fromsp.JCM2471, the gene encoding a new glycerol kinase and (ii) a method for preparing the glycerol kinase by gene recombination technique, A Glycerol kinase which has high resistance against preservative, a recombinant vector comprising a gene encoding the glycerol kinase, a transformant prepared by transforming a host cell with the recombinant vector, and a method for producing the glycerol kinase, including culturing the transformant to produce glycerol kinase, and collecting the resulting glycerol kinase.

Glycerol kinase, which has high resistance against preservative

It is intended to provide a method of conveniently screening a novel protein or a novel partial sequence protein having a blocking ability based on amino acid sequence data; and a protein achieving an improved blocking efficiency that can be expressed on a large scale in. A method of screening a novel protein or a novel partial sequence protein having a blocking ability based on amino acid sequence data; a protein characterized by achieving an improved blocking efficiency owing to an amino acid sequence modification; and a method of utilizing the protein.

Protein achieving improved blocking efficiency

The present invention relates to modified pyrroloquinoline quinone dependent glucose dehydrogenase (PQQGDH) having lower activity with respect to disaccharides and/or greater stability than wild-type PQQGDH.

Modified pyrroloquinoline quinone (PQQ) dependent glucose dehydrogenase with superior substrate specificity and stability

A protein having been modified by addition, deletion, insertion or substitution of at least one amino acid in an amino acid sequence constituting a protein having a sarcosine oxidase activity and still having the sarcosine oxidase activity, characterized by having an improved stability in the state of a liquid compared with the unmodified one and/or having a lowered action on L-proline compared with the unmodified one. A sarcosine oxidase having at least one of the following characteristics, i.e., an action on L-proline being 0.7% or less based on sarcosine and a Km value to L-proline being 150 mM or more, when measured at 37&#xb0; C. and pH 8.0; a process for producing sarcosine oxidase having an excellent substrate specificity which comprises culturing a microorganism capable of producing sarcosine oxidase and collecting the sarcosine oxidase from the culture medium; and a reagent for measuring creatinine which contains the sarcosine oxidase.

Modified sarcosine oxidase, process for producing the same and reagent composition using the same

A creatine amidinohydrolase having the following physicochemical properties:

Creatine amidinohydrolase, production thereof and use thereof

The present invention provides a stable lyophilized PQQ-dependent glucose dehydrogenase composition comprising a PQQ-dependent glucose dehydrogenase together with (i) at least one compound selected from the group consisting of aspartic acid, glutamic acid, &#x3b1;-ketoglutaric acid, malic acid, &#x3b1;-ketogluconic acid, &#x3b1;-cyclodextrin and their salts and (ii) an albumin.

Stable PQQ-dependent glucose dehydrogenase composition

The present invention provides novel glutathione-dependent formaldehyde dehydrogenase that makes possible quantitative measurement of formaldehyde by cycling reaction, and a determination method of formaldehyde and biological components, such as creatinine, creatine, and homocysteine, which produces formaldehyde as a reaction intermediate. In addition, the present invention provides a reagent kit for the above-mentioned determination method. The present invention provides a novel determination method of a homocysteine using transferase utilizing homocysteine and other compound as a pair of substrates. Particularly, the present invention provides a determination method of homocysteine which includes bringing betaine-homocysteine methyltransferase and dimethylglycine oxidase into contact with a sample and measuring produced hydrogen peroxide or formaldehyde. Moreover, the present invention provides novel dimethylglycine oxidase stable to thiol compound, which is suitably used for the measurement. The present invention provides a reagent kit used for any of the above-mentioned determination methods of homocysteine.

Determination method of biological component and reagent kit used therefor

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Tsuruga, Japan

Atsushi Sogabe