Takara Shuzo Co., Inc.

Nagahama Bio-laboratory Incorporated

Education Corporation Kansai Bunri Sougougakuen

Atsushi Oshima

Ikunoshin Kato

Hiroaki Sagawa

Harumi Ueno

Hirofumi Yoshioka

Yoshinobu Okuno

Fumie Yusa

Takashi Takabatake

An ink, which contains DNA and is hardly decomposed by an external stimulus such as ultraviolet light, heat, an acid or an alkali, is provided. A method of easily analyzing a DNA in an ink composition is also provided. An ink composition containing a DNA and having a water-tolerance at a certain level or higher is prepared. The DNA in a print that is produced by using the above ink composition is quickly extracted with water or an aqueous solution and analyzed. Furthermore, a DNA in a print that is produced by using an oil- and water-based ink composition containing the DNA is quickly extracted with water or an aqueous solution and analyzed.

DNA-containing ink composition

A plasmid vector characterized by comprising a promoter sequence that can be recognized by an RNA polymerase which is not inherent in a host and that controls the expression of desired genes and a replication origin that increases the number of copies under the induction by exogenous factors; methods for expression and isolation of target genes by using the vector; a polypeptide having the activity of an AccIII restriction endonuclease; and a DNA encoding the polypeptide. The invention provides for the first time a plasmid vector which can introduce an exogenous desired gene encoding proteins which are lethal or harmful to hosts into the hosts, a method for efficiently expressing the proteins by using the vector, and also a method for permitting a restriction endonuclease-gene constituting a restriction-modification system to be isolated even in the absence of a modification enzyme gene, which has been difficult in the prior arts.

Gene isolation method

Plasmid

An isolated DNA is provided coding for the variable region of an anti-human influenza A type virus antibody having the following characteristics: (a) specifically binds to the stem region of haemaggulutinin molecule of H1N1 and H2N2 subtypes of human influenza A type virus but does not specifically bind to the stem region of haemaggulutinin molecule of H3N2 subtype; and (b) has a neutralization activity for the H1N1 and H2N2 subtypes of human influenza A type virus but no neutralization activity for the H3N2 subtype.

DNA coding for variable region to human influenza A type virus

Growing community of inventors

Otsu, Japan

Atsushi Oshima