Riken Corporation

Sumitomo Chemical Company, Limited

Sumitomo Dainippon Pharma Co., Ltd.

Sumitomo Pharma Co., Ltd.

Kyoto University

Racthera Co., Ltd.

Atsushi Kuwahara

Yoshiki Sasai

Masayo Takahashi

Suguru Yamasaki

Yasushi Hiramine

Yoshiki (deceased) Sasai

Jun Takahashi

Mototsugu Eiraku

Daisuke Doi

Hideya Sakaguchi

Satoru Okuda

Daiki Nukaya

Kenji Yoshida

The present invention provides a method for producing neural cells or a neural tissue, including the following steps (1)-(3): (1) a first step of culturing pluripotent stem cells in the absence of feeder cells and in a medium containing 1) a TGFβ family signal transduction pathway inhibiting substance and/or a Sonic hedgehog signal transduction pathway activating substance, and 2) a factor for maintaining undifferentiated state,(2) a second step of culturing the cells obtained in the first step in suspension to form a cell aggregate, and(3) a third step of culturing the aggregate obtained in the second step in suspension in the presence or absence of a differentiation-inducing factor to obtain an aggregate containing neural cells or a neural tissue.

Production method for nerve tissue

The present invention provides a method for producing retinal cells or a retinal tissue, comprising the following steps (1)-(3):

Production method for retinal tissue

A cell population comprising Corin- and/or Lrtm1-positive cells was produced by the following steps (1) and (2), from which Corin positive and/or Lrtm1 positive cells are collected using a substance that binds to Corin and/or a substance that binds to Lrtm1, and dopaminergic neuron progenitor cells are produced by performing suspension culture of the Corin positive and/or Lrtm1 positive cells in a culture solution containing one or more nutritional factors:

Method for producing dopaminergic neuron progenitor cell

The invention provides a method for producing a ciliary marginal zone stem cell induced to differentiate from a pluripotent stem cell, including either the following step (1) or step (2), or both of these steps: (1) a step of floating culturing cells obtained from a cell aggregate containing a ciliary marginal zone-like structure induced to differentiate from pluripotent stem cells, thereby obtaining a retinosphere; and (2) a step of collecting stage specific embryonic antigen-1 positive cells from cells obtained from a cell aggregate containing a ciliary marginal zone-like structure induced to differentiate from pluripotent stem cells.

Method for manufacturing ciliary margin stem cells

The present invention provides a method for producing a retinal cell or a retinal tissue, including the following steps (1)-(3):

Method for producing retinal tissue

The present invention provides a method for producing neural cells or a neural tissue, including the following steps (1)-(3):

The present invention provides a method for producing a cell aggregate containing a ciliary marginal zone-like structure, including a step of culturing a cell aggregate containing a retinal tissue in which Chx10 positive cells are present in a proportion of 20% or more and 100% or less of the tissue in a serum-free medium or serum-containing medium each containing a substance acting on the Wnt signal pathway and a substance inhibiting the FGF signal pathway for only a period before the appearance of a RPE65 gene expressing cell, followed by culturing the 'cell aggregate in which a RPE65 gene-expressing cell does not appear' thus obtained in a serum-free medium or serum-containing medium each free of a substance acting on the Wnt signal pathway and so on. According to the production method of the present invention, a ciliary marginal zone-like structure can be produced with high efficiency.

Method for manufacturing ciliary marginal zone-like structure

A specific region such as a continuous epithelial structure is automatically detected in a cell aggregate. A region detecting method includes an input step (S01) of inputting an image obtained by imaging a cell aggregate, an outline detecting step (S02) of detecting an outline of the cell aggregate together with an order along the outline from the input image, a region detecting step (S03) of detecting a specific region included in the cell aggregate based on the detected outline and order, and an output step (S04) of outputting information indicating the detected region.

Growing community of inventors

Kobe, Japan

Atsushi Kuwahara