Hitachi, Ltd.

Hitachi-High-Technologies Corporation

Atsumu Hirabayashi

Hideaki Koizumi

Yasuaki Takada

Minoru Sakairi

Kinya Kobayashi

Kiyomi Yoshinari

Yukiko Hirabayashi

Toshiyuki Yokosuka

Yasushi Terui

Takayuki Nabeshima

Akihiko Okumura

Min Huang

Kaoru Umemura

Izumi Waki

Kisaburo Deguchi

Kenko Uchida

Akihiro Sano

Masako Ishimaru

Yuichiro Hashimoto

Yoichi Ose

Hiroyuki Satake

Takashi Baba

Tadao Mimura

Atsushi Otake

Atsushi Ootake

Masahiro Yamaoka

Atsushi Ohtake

Hiroki Ito

Youichi Ose

A mass spectrometric device of the present invention includes a quadrupole filter () located upstream of a quadrupole ion trap () and configured to transmit ions in a predetermined filter range, and determines the filter range of the quadrupole filter () such that accumulation time for the ions in the quadrupole ion trap () is maximized. The accumulation time for the ions is determined based on mass spectrometry data information. With this configuration, the present invention produces advantageous effects of improving analysis throughput and an S/N ratio in an analysis of a minor sample component mixed in various accompanying components by using the mass spectrometric device using the quadrupole ion trap.

Mass spectrometric system

During the structural analysis of a protein or peptide by tandem mass spectroscopy, a peptide ion derived from a protein that has already been measured and that is expressed in great quantities is avoided as a tandem mass spectroscopy target. A peptide derived from a minute amount of protein, which has heretofore been difficult to analyze, can be automatically determined as a tandem mass spectroscopy target within the real time of measurement. Data concerning a protein that has already been measured and a peptide derived from the protein is automatically stored in an internal database. The stored data is collated with measured data with high accuracy to determine an isotope peak. In this way, the process of selecting a peptide peak that has not been measured as the target for the next tandem analysis can be performed within the real time of measurement and a redundant measurement of peptides derived from the same protein can be avoided. The information contained in the MSspectrum is effectively utilized in each step of the MSinvolving a multi-stage dissociation and mass spectroscopy (MS), so that the flows for the determination of the next analysis content and the selection of the parent ion for the MSanalysis, for example, can be optimized within the real time of measurement and with high efficiency and accuracy. Thus, a target of concern to the user can be subjected to tandem mass spectroscopy without wasteful measurement.

Mass spectrometer system

To analyze glycans and peptide sequences without liberating glycans from glycosylated peptides, a glycan structure is analyzed through negative-ion CID, in which sialic acid and fucose are resistant to elimination, and a peptide sequence is analyzed through positive-ion ECD.

Methods and instruments for identification of glycosylated proteins and peptides

An ion source includes a body having a gas passage and an orifice. A capillary is inserted into the gas passage so that a tip portion of the capillary extends into the orifice. A gas supplier supplies a gas into the gas passage to form a gas flow through the gas passage along the capillary and through the orifice past a tip of the capillary so that the gas flow sprays a sample solution flowing through the capillary from the tip of the capillary. A flow controller regulates a pressure of the gas in the gas passage to adjust a characteristic value F/S to a predetermined value, where F is a flow rate of the gas flow at standard conditions (20&#xb0; C., 1 atmosphere), and S is a difference between a cross section of the orifice and a cross section of the tip portion of the capillary in the orifice.

Ion source and mass spectrometer instrument using the same

The present invention can provide a mass spectrometric system judging whether a measurement target is a substance required by an operator within an actual measurement time, when a substance (particularly such as protein or sugar chains) is analyzed. In the mass spectrometric system using a tandem mass spectrometer, a particular substance obtained by separating a sample is ionized, and mass analysis of the ionized substance is performed to obtain a spectrum. This spectrum is compared with a particular spectrum stored in advance, to thereby determine whether both the spectra match with each other. When a match is determined, a particular ion is further ionized within a particular time for detailed analysis. The invention also provides a mass spectrometric method, a diagnosis system and an inspection system each using the mass spectrometric system, and a program for operating a computer to control those systems with desired functions.

Mass spectrometric method, mass spectrometric system, diagnosis system, inspection system, and mass spectrometric program

The present invention provides a tandem type mass analysis system capable of carrying out the differential analysis with high efficiency by the tandem type mass analysis. A predetermined number of m/z regions are set up for carrying out the mass analysis with the all ions included therein being dissociated collectively for each m/z region so as to obtain measurement MSdata. By comparing the measurement MSdata with reference MSdata stored in a reference data base, a difference thereof is detected. For the m/z region with a differential component detected, the mass analysis is carried out collectively without dissociation for the all ions included therein so as to obtain measurement MSdata. By comparing the measurement MSdata with the reference MSdata, a difference thereof is detected. From the difference thereof, a parent ion considered to be the differential component factor is presumed for carrying out the mass analysis with the same being dissociated.

Tandem type mass analysis system and method

An object of the present invention is to provide a mass spectrometry system capable of improving an efficiency of obtaining information on a structure of substances, shortening a time taken for a measurement and substance identification, and improving identification accuracy. The system comprises: a process of mass analyzing an ionized analyte; a first fragmentation process where a first ion is selected from the ions observed in a mass spectrometry to fragment it; a process of mass analyzing a plurality of the ions generated in the first fragmentation process; a process of determining fragment ion combination capable of reconstructing the first ion using a result of the mass spectrometry; a second fragmentation process where the fragment ions contained in the fragment ion combination are fragmented; and a process of mass analyzing the fragment ions generated in the second fragmentation.

Method of identifying substances using mass spectrometry

In a method of measuring by a tandem mass spectrometer a sample labeled with an isotope, measuring throughput is improved. In a technique in which tandem mass spectrometer is used to analyze a sample labeled with an isotope, spectra obtained by a first-stage measurement (MS) are analyzed during a measuring session in a realtime fashion to determine ions to be used in second-stage and subsequent dissociation&#x2022; spectral measurement (MS2).

Mass spectrometry and mass spectrometry system

A mass spectrometric apparatus of high sensitivity, including a spray ionization interface suitable for the ionization of a low flow-rate liquid that prevents charged particles from being introduced into a vacuum device; wherein the ion source comprises a capillary having a first end having an inner diameter that gradually reduces in size in the direction of gas flow and wherein a liquid sample is introduced into an opposite second end of the capillary; a gas guide tube which guides gas flow along an outer periphery of the first end the capillary and which sprays the liquid sample from the first end of the capillary; and a gas introducing section for introducing the gas into the gas guide tube. A first end of the gas guide tube has a reduced inside diameter and receives the first end of the capillary in a holding member. Gaseous ions produced are introduced into a vacuum section through an ion intake port and are subjected to mass separation by a mass spectrometer. The angle between the central axis of the capillary and that of the ion intake port is greater than about 15&#xb0;.

Ion source and mass spectrometric apparatus

According to the existing mass spectrometric system, whether or not the informations are sufficient for analyzing substances (particularly proteins, sugars, etc.) cannot be judged in the process of measurement. Further, it is difficult to find out isomers having just the same mass number or compounds very close in mass only from the MS data. According to this invention, whether or not the retention time in the LC (or GC) of peptide formed at the time of enzymatic decomposition of protein coincides with the predicted retention time assumed from the amino acid sequence predicted from MSmass spectrometry data is judged within the actual time period of measurement, and thereby the quality of MSmass spectrometry data (quantity of information) is judged.

Mass spectrometry system

The invention relates to an LC/MS interface provided with structure that the end of a spray capillary is rarely damaged and the object is to provide the LC/MS interface which a general user can easily operate, which inhibits the extension of a separation band and the deterioration of detection sensitivity and which enables high separation and sensitive analysis. A metallic member having a hole for connecting a separation column and a spray capillary and a resin member for fixing the upstream side of the short spray capillary are used. Further, to enhance operability, spray ionization using gas spraying is used.

Mass spectrometer

The measurement throughput and the precision in sample identification are improved in a tandem type mass spectrograph. Thus, in a mass spectrum analyzing system utilizing a tandem type mass spectrograph in which the selection of an ionic species to serve as the measurement target, dissociation thereof and spectral measurement are repeated in n stages, the ionic species to be measured in MSis selected based on the mass-to-charge ratios (m/z values) obtained as a result of the spectral analysis in MS(n&#x2267;2), and this procedure is repeated until the sequence of a required number of amino acids is determined.

Mass spectrum analyzing system

A practical mass spectrometer for proteome analysis is provided. In an ion trap-connected, orthogonal acceleration type time-of-flight mass spectrometer, the mass-to-charge ratio range that may be analyzed by one procedure is increased by providing means for reducing the velocity of ions ejected from an ion trap. The efficiency in protein identification in proteome analysis is thereby improved.

Mass spectrometer and measurement system using the mass spectrometer

The capillary electrophoresis system includes a wafer-shaped part having passages filled with a buffer solution for introducing sample solutions; a body having a configuration suitable to removably hold and to move the wafer-shaped part. The body includes first and second electrodes for applying a voltage across both ends of passages of the wafer-shaped part to separate and remove the sample solution. The body also includes first and second buffer reservoirs conductive to passages of the wafer-shaped part at specific positions for filling buffer solution around the first and second electrodes. The system according to the present invention facilitates washing of electrophoresis passages and reduces the time and labor costs required for replacement of the fused-silica wafers and simplifies operation by allowing a plurality of sample solutions to be analyzed more quickly.

Growing community of inventors

Kodaira, Japan

Atsumu Hirabayashi