Sumitomo Rubber Industries Limited

Kao Corporation

Jcr Pharmaceuticals Co., Ltd.

Nitto-denko Corporation

Other

Fuji Latex Co., Ltd.

Atsuko Kawasaki

Toshiaki Sakaki

Takefumi Nakashita

Atsushi Sugimura

Kazutoshi Mihara

Yoshiharu Inui

Yasuyuki Tanaka

Masaharu Hayashi

Kazuhiko Shibata

Shinichi Nakade

Eiji Kanamaru

Tsuyoshi Fukui

Yukichi Hatano

Shinichi Hori

Miyo Miki

Toshimichi Shirouzu

Masahiro Asano

Shoichiro Kamei

Yae Ito

Yuukichi Hatano

Kouta Ootsuki

Toshihiro Yagi

Keisuke Mukai

Disclosed is a method for production of recombinant human alpha-galactosidase A (rh alpha-Gal A) in a large scale, with a high purity. The method comprises the steps of (a) culturing rh alpha-Gal A-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to anion-exchange column chromatography, (d) to hydrophobic column chromatography, (e) to a column chromatography employing as solid phase a material having affinity for phosphate group, (f) to cation-exchange column chromatography, (g) to dye-affinity column chromatography, and (h) to gel filtration column chromatography, in the order.

Method for production of recombinant human alpha-galactosidase A from material containing contaminant host cell proteins by chromatography

Disclosed is a method for production of recombinant human alpha-galactosidase A (rh alpha-Gal A) in a large scale, with a high purity. The method comprises the steps of (a) culturing rh alpha-Gal A-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to anion-exchange column chromatography, (d) to hydrophobic column chromatography, (e) to a column chromatography employing as solid phase a material having affinity for phosphate group, (f) to cation-exchange column chromatography, and (g) to gel filtration column chromatography, in the order.

Method for production of recombinant human alpha-galactosidase A

Disclosed is a method for production of recombinant human iduronate 2-sulfatase (rhI2S) (the 26th to 550th amino acids of SEQ ID NO: 10) in a large scale, with a high purity, and mannose 6-phosphate residues. The method comprises the steps of (a) culturing rhI2S (the 26th to 550th amino acids of SEQ ID NO: 10)-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to cation-exchange column chromatography, (d) to dye affinity column chromatography, (e) to anion-exchange column chromatography, and (f) to a column chromatography employing as solid phase a material having affinity for phosphate group, and (g) to gel filtration column chromatography, in the order.

Method for production of recombinant human iduronate 2-sulfatase

A process is disclosed, by which protein can be removed easily and efficiently from natural rubber latex so that deproteinized natural rubber latex can be produced with high productivity and low cost. Particularly, a process for the production of deproteinized natural rubber latex is disclosed, which comprises treating natural rubber latex with a proteolytic enzyme and a surfactant to decompose protein in natural rubber latex, washing the treated natural rubber latex in the presence of a salt. According to the process, deproteinized natural rubber latex useful as a counter-allergy material having stable qualities can be produced easily with high productivity and low cost.

Process for producing deproteinized natural rubber latex

Described is a process for the production of depolymerized natural rubber, which makes it possible to obtain, at high reaction efficiency, of depolymerized natural rubber in the liquid form and having a narrow molecular weight distribution; and also a process for the production of depolymerized natural rubber which is free from odor or coloring peculiar to natural rubber and is also free from the danger of immediate allergy derived from protein. The process according to the present invention comprises adding a carbonyl compound to a natural rubber latex, and then subjecting the resulting natural rubber to air oxidation in the presence of a radical forming agent or adding a carbonyl compound to the latex of deproteinized natural rubber and then subjecting the deproteinized natural rubber to air oxidation optionally in the presence of a radical forming agent.

Production process of depolymerized natural rubber

A depolymerized natural rubber having a number-average molecular weight of from 2,000 to 50,000 obtained by the depolymerization of a deproteinized natural rubber. A process for the preparation of a depolymerized natural rubber comprises dissolving a deproteinized natural rubber into an organic solvent so as to give a concentration of from 1 to 30% by weight and then carrying out air oxidation of the resulting solution in the presence of a metallic catalyst. The depolymerized natural rubber can provide the same physical properties as ordinary vulcanized rubbers, exhibits no odor or color characteristic to natural rubbers and causes no immediate allergy induced by protein.

Depolymerized natural rubber and process for preparation thereof

This invention relates to a compressive hemostatic belt wherein a cup-shaped rigid case is attached by an attaching device to a predetermined position on a band made of non-stretchable or low-stretchable fiber, nonwoven fabric or film, and a balloon adapted to be inflated by being filled with fluid is received in the rigid case. Preferably, at least the portion of the band which corresponds to the leg when the band is wrapped around the body is shaped in curve form. Buckles may be attached to the opposite ends of the band and a buckle attaching plate may be attached to the rigid case. One end of the band may be provided with projections so that when the band is wrapped around the body, the other end of the band is thrusted on the projections to fix the band in position. If the band is made of adhesive material, more reliable fixing can be achieved. A check valve adapted to prevent fluid from flowing back from the balloon may be removably connected to the balloon to provide for reuse of the check valve.

Compressive hemostatic belt

The invention presents a compressive hemostatic belt which is easy to handle and is disposable. A balloon 5 inflatable by filling with fluid is mounted on a specific position of a strip 1 made of non-elastic or low-elastic fiber cloth or the like through mounting means, and a pump 8 and a pressure gauge 9 are connected to the balloon 5 through a check valve 6. The balloon 5 is filled with a fluid by manipulating the pump 8 while observing the pressure gauge 9 to inflate the balloon 5, so that only the area where bleeding is to be stopped is put under pressure. Since the balloon 5 is inflated only on the specified area, a piece of hard material 4 may be placed between the balloon 5 and the strip 1. A non-elastic reinforcing member 11 inserted on the opposite side of the balloon 5 can be varied, or the confronting portion of the strip 1 against the balloon 5 can be formed of a non-elastic reinforcing member stronger than the materials of other portions of the strip.

A compressive hemostatic belt which is easily operable and thrown away after use, incorporates a strip (1) in the form of a nonstretch or low-stretch textile fabric, and a bag (5) expansible by being filled with fluid that is attached to the strip at a predetermined position by an attaching device. A pump (8) and a pressure gauge (9) are connected to the bag (5) attached at the predetermined position on the strip (1), so that the bag is expanded by operating the pump so as to fill the bag (5) with a suitable fluid while watching the pressure gauge (9). The bag thus presses down on an area (A) where bleeding is to be stopped.

Growing community of inventors

Hyogo, Japan

Atsuko Kawasaki