Lunaphore Technologies Sa

Ecole Polytechnique Federale De Lausanne

Other

Ecole Polytechnique Féderale De Lausanne (epfl)

Ata Tuna Ciftlik

Diego Gabriel Dupouy

Martin Gijs

Pierre Joris

Marco Ammann

Marta Comino

Giulia Cappi

Haluk Kulah

Deborah Heintze

Deniz Eroglu

Déborah Heintze

The invention relates to a device and method for in situ temperature-induced antigen retrieval of samples wherein all steps are performed under a pressure higher than the atmospheric pressure on a sample immobilized on a sample support which can be further subjected to staining and imaging on the same sample support, optionally by cycle multiplexing that enables imaging of various molecular targets through multi-molecular read-outs on the same sample in a rapid, highly sensitive and reliable manner.

Methods of in situ antigen retrieval of a biological sample and imaging thereof

Microfluidic network device () configured to supply reagents to a biological tissue sampling device (), comprising a plurality of microfluidic inlet channels () connected to respective sources of said reagents, at least one common outlet channel (), and a plurality of valves () interconnecting an outlet end () of each of said plurality of inlet channels to said at least one common outlet channel.

Microfluidic network device

Microfluidic cartridge () comprising a sampling device () having a sealing ring () arranged to form a microfluidic chamber () when a support containing a biological sample is brought into contact with the sealing ring, and a microfluidic network device () configured to supply reagents to the microfluidic chamber. The sampling device further comprises inlet and outlet distribution networks () in fluid communication with the microfluidic chamber and a slide holder () to guide and position said support containing a biological sample on the sampling device. The microfluidic network device comprises a plurality of reagent inlet channels () fluidly connectable to reagent sources, at least one reagent outlet channel () fluidly connected to the sampling device inlet distribution network (), and a plurality of valves () operable to selectively connect the inlet channels to the at least one outlet channel. The sampling device () and microfluidic network device () are formed on a common microfluidic support () as a single part.

Microfluidic cartridge with built-in sampling device

The invention relates to a method for in situ imaging of samples by cycle multiplexing that enables imaging of various molecular targets through multi-molecular read-outs on the same sample in a rapid, highly sensitive and reliable manner. The invention is further related to imaging buffers preventing the degradation of the sample and of the imaging reagents, which are particularly useful in a method of in situ imaging of samples by cycle multiplexing according to the invention.

Methods of sample cycle multiplexing and in situ imaging

The invention relates to a method for quantitative measurement of a biomarker by in situ immunofluorescence and uses thereof. In particular, the invention relates to a method which is a useful tool for use in the field of diagnosis, prevention and/or treatment of disease or disorders, in particular in the field of cancer management and therapy.

Method for quantitative measurement of a biomarker by in situ immunofluorescence and uses thereof

A biological and chemical sample processing device that b. comprises a high pressure-resistant, shallow and wide area microfluidic chamber having at least one wall formed by a detachable slide containing samples such as immobilized entities, biological samples or molecules, c. comprises an arrangement of microfluidic access holes for injecting to and collecting fluid form said chamber, d. is interfaced with inlet ports and microfluidic channels which are formed external to the chamber, e. is configured so that the slide may be brought into contact with the device to form the said chamber, f. is adapted to deliver and to transport fluidic substances and reagents inside said chamber in a fast manner, preferably within less than 15 seconds, and in a regular or uniform way owing to said arrangement of microfluidic access holes.

Sample processing device with detachable slide

This dielectrophoretic micro cell chromatography device with concentric electrodes and spiral microfluidic channels, produced according to MEMS technology subject to this invention; is composed of 4 groups of effect electrodes, inlet electrodes, spiral zone and central span, having exterior upper electrode (), interior sub electrode with 3D geometry (), upper inlet electrode (), sub inlet electrode (), spiral zone (), central span (), constant reading point and Insulating wafer () as the main components.

Dielectrophoretic cell chromatography device with spiral microfluidic channels and concentric electrodes, fabricated with MEMS technology

Laterally oscillating gravimetric sensing device embeddable under micro-fluidic channels and fabricated with micro-electro mechanical systems (MEMS) technology, which detects biological cells and analytes by measuring the change of mass attached on its surface is composed of four main groups, namely a resonator that can be placed onto the basis of the channel, components of the resonator bio-activation, a micro fluidic channel, and the microfabrication techniques, and its main components are the proof mass (), comb fingers fixed to proof mass (), folded spring beams (), channel floor and mechanical soil (), stationary electrodes (), comb fingers attached to the stationary electrodes (), golden film deposited onto the mass (), immobilized biologic recognition molecules (), and micro fluidic channel placed on resonator structure ().

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Morges, Switzerland

Ata Tuna Ciftlik