Bioneer A/s

Bioteknologisk Institut

Chr Hansen's Laboratorium Danmark A/s

Chr. Hansen A/s

Astrid Vrang

Hans Israelsen

Peter Ravn

Soeren Michael Madsen

Lars Bredmose

Jose Arnau

Eric Johansen

Dan Nilsson

Egon Bech Hansen

Søren Michael Madsen

Mads Gronvald Johnsen

Anne Cathrine Steenberg

Mads Groenvold Johnsen

José Arnau

Mads Groenvald Johnsen

Soren Michael Madsen

The invention provides a fed-batch or continuous process for producing heterologous peptides, polypeptides or proteins in lactic acid bacteria, comprising the steps of (i) constructing a recombinant lactic acid bacterium comprising a nucleotide sequence coding for the heterologous peptide, polypeptide or protein, and appropriate regulatory nucleotide sequences such as regulatable promoters and signal peptides to control the expression of the coding sequence and the secretion of gene product, (ii) cultivating the recombinant bacterium under or continuous cultivation conditions to express the gene, and (iii) harvesting the recombinant bacterium or the gene product. Preferably, the cultivation medium is a chemically defined or synthetic medium optionally supplemented with yeast extract.

Fermentation method for production of heterologous gene products in lactic acid bacteria

A method of identifying nucleotide sequences coding for signal peptides in lactic acid bacteria, using a DNA molecule comprising a transposon including a promoterless reporter gene from which DNA molecule a region between the LR and the reporter gene is deleted and the DNA molecule comprises a DNA sequence coding for a secretion reporter molecule. By deleting the region between the LR and the reporter gene, stop codons in-frame with the secretion reporter molecule is removed which upon transposition permits translational fusions from upstream the LR.

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Lactic acid bacterial regulatable expression system

Recombinant lactic acid bacterium containing an inserted promoter and

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Helsinge, Denmark

Astrid Vrang