Biogen Ma Inc.

Biogen Idec Ma Inc.

Asif Ladiwala

Jorg Thommes

Joydeep Ganguly

John Pieracci

Paul Cunnien

Basav Ghosh

Robert Song

Methods and kits for regenerating antibody binding resins are provided. In some embodiments the methods include a first step of washing an antibody binding resin with a reducing solution, followed by a second step of washing the antibody binding resin with a chaotropic solution.

Regeneration of chromatography material

The present invention provides automated systems and methods for monitoring column performance in process chromatography, and applications thereof. In an embodiment, column performance is monitored by generating a plurality of process values such as, for example, conductivity values or pH values with a detector during a chromatography step transition between a first mobile phase liquid and a second mobile phase liquid. The process values are transformed to form transformed process values in which noise present in the process values is suppressed. Column performance parameters are calculated based on the transformed process values and displayed during movement of the second mobile phase liquid through the chromatography column. The displayed performance parameters enable an operator to make a determination, for example, regarding the quality of the chromatography column packing and whether to continue the chromatography process or stop the chromatography process until the chromatography column can be repacked or replaced.

Automated system and method for monitoring chromatography column performance, and applications thereof

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San Diego, CA, United States of America

Asif Ladiwala