Idexx Laboratories, Inc.

Ashok P Aiyappa

Robert L Lawton

Hongliang Guo

Eugene Regis Krah, Iii

Brion Mermer

Wendy W Liu

The present invention provides canine heavy chain and light chain (both &#x3bb; and &#x3ba;) variable domain nucleic acids and polypeptides. The present invention also provides caninized antibodies comprised of the presently disclosed canine variable domain framework sequences and CDRs specific for an antigen obtained from antibodies from other than canines (preferably mouse). The invention also provides methods of making caninized antibodies and using them. This disclosure specifically describes caninized antibodies that are useful in the treatment of canine allergy. The general process of caninization permits the development of useful therapeutics, which includes but is not limited to allergy, osteosarcomma's and lymphomma's.

Methods for using canine immunoglobulin variable domains and caninized antibodies

The present invention provides the nucleotide and amino acid sequences of canine IL-5. It also sets forth a recombinant DNA molecule, and conservative variants thereof, comprising the DNA sequence of canine IL-5, and recombinant vectors such as expression vectors, comprising a DNA sequence encoding canine IL-5. Further, the invention sets forth polypeptides produced using a recombinant expression vector containing a canine IL-5 sequence. Also disclosed are compositions comprising a nucleotide sequence that is antisense to a DNA sequence encoding a component of canine IL-5, wherein the component can be the full canine IL-5 protein. Disclosed are a cloning vector comprising a DNA sequence of canine IL-5 cDNA material; a recombinant cell line comprising a DNA sequence of the canine IL-5 cDNA material; a method for producing a polypeptide comprising a step of expressing a peptide comprising an amino acid sequence encoded by a cloning vector comprising canine IL-5 cDNA material; and a cell that expresses a recombinant polypeptide encoded by a DNA sequence (or conservative variants thereof) that encodes canine IL-5 or components thereof. Furthermore, antibodies that bind to canine IL-5, and mimetopes of IL-5 epitopes are also disclosed.

Methods and compositions concerning canine interleukin 5

The present invention provides methods and compositions for decreasing IgE levels in dogs. The methods and compositions are useful for treating allergic symptoms in dogs. The invention may comprise chimeric canine anti-IgE monoclonal antibody compositions and methods for using the compositions in the treatment of allergy. In preferred embodiments, the compositions of the present invention may act by binding soluble IgE in plasma, or by inhibiting IgE from binding to receptors on mast cells, B cells, and basophils.

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Scarborough, ME, United States of America

Ashok P Aiyappa