Harvard College

The Brigham and Women's Hospital, Inc.

Children's Medical Center Corporation

Ashok A Kumar

George M Whitesides

Charles R Mace

Dyann F Wirth

Nathan D Shapiro

Ozge Akbulut Halatci

Carlo Brugnara

Thomas P Stossel

Matthew Reiser Patton

Caeul Lim

Yovany Moreno

Manoj T Duraisingh

Si-yi Ryan Lee

Gaetana D'Alesio-Spina

Jonathan W Hennek

A multi-phase system includes a phase-separated solution comprising at least two phases, each phase having a phase component selected from the group consisting of a polymer, a surfactant and combinations thereof, wherein at least one phase comprises a polymer, wherein the phases, taken together, represent a density gradient. Novel two-phase, three-phase, four-phase, five-phase, or six-phase systems are disclosed. Using the disclosed multi-phase polymer systems, particles, or other analyte of interest can be separated based on their different densities or affinities.

Multiphase systems and uses thereof

The disclosed methods use a multi-phase system to separate samples according to the density of an analyte of interest. The method uses a multi-phase system that comprises two or more phase-separated solutions and a phase component such as a surfactant or polymer. The density of the analyte of interest differs from the densities of the rest of the sample. The density of the analyte of interest is substantially the same as one or more phases. Thus, when the sample is introduced to the multi-phase system, the analyte of interest migrates to the phase having the same density as the analyte of interest, passing through one or more phases sequentially.

Density-based separation of biological analytes using mutliphase systems

A kit for separating a sample comprising one or more biological analytes of interest using a multi-phase system comprising: a) two or more phase components selected from the group consisting of a polymer, a surfactant, and combinations thereof; b) optionally a tag molecule capable of binding the one or more biological analytes of interest; and c) instructions for: (i) combining the two or more phase-separated solutions with a common solvent to create a multi-phase system; (ii) optionally, combining the biological analyte of interest and tag molecule, and (iii) separating the biological analyte of interest from the sample.

Kit for density-based separation of biological analytes using multiphase systems

An aqueous multi-phase system for diagnosis of sickle cell disease is described, including two or more phase-separated phases including: a first aqueous phase including a first phase component and having a first density between about 1.025 g/cmand about 1.095 g/cm; and a second aqueous phase including a second phase component and having a second density between about 1.100 g/cmand about 1.140 g/cm; wherein the first density is lower than the second density; and each of the first and second phase components include at least one polymer.

Multiphase systems for diagnosis of sickle cell disease

Density-based separation of biological analytes using multiphase systems

Growing community of inventors

Medford, MA, United States of America

Ashok A Kumar