Integrated Dna Technologies, Inc.

Walder, Joseph Alan

Behlke, Mark Aaron

Owczarzy, Richard

Lennox, Kimberly Ann

Jacobi, Ashley Mae

Ashley Mae Jacobi

Mark Aaron Behlke

Michael Allen Collingwood

Garrett Richard Rettig

Mollie Sue Schubert

Joseph Alan Walder

Richard Owczarzy

Kimberly Ann Lennox

This invention pertains to modified compositions for use in CRISPR systems, and their methods of use. In particular, length-modified and chemically-modified forms of crRNA and tracrRNA are described for use as a reconstituted guide RNA for interaction with Cas9 of CRISPR systems. The resultant length-modified and chemically-modified forms of crRNA and tracrRNA are economical to produce and can be tailored to have unique properties relevant to their biochemical and biological activity in the context of the CRISPR Cas9 endonuclease system.

CRISPR-based compositions and methods of use

This invention pertains to modified compositions for use in CRISPR systems, and their methods of use. In particular, length-modified and chemically-modified forms of crRNA and tracrRNA are described for use as a reconstituted guide RNA for interaction with Cas9 of CRIPSR systems. The resultant length-modified and chemically-modified forms of crRNA and tracrRNA are economical to produce and can be tailored to have unique properties relevant to their biochemical and biological activity in the context of the CRIPSR Cas9 endonuclease system.

The invention pertains to modifications for antisense oligonucleotides, wherein the modifications are used to improve stability and provide protection from nuclease degradation. The modifications could also be incorporated into double-stranded nucleic acids, such as synthetic siRNAs and miRNAs.

Modifications for antisense compounds

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Tiffin, IA, United States of America

Ashley Mae Jacobi