Fuji Photo Film Company, Limited

Fuji Photo Film, Inc.

Fuji Shashin Film Kabushiki Kaisha

Asaji Kondo

Masao Kitajima

Fuminori Arai

Kenichiro Yazawa

Harumi Katsuyama

Shizuo Miyano

Osamu Seshimoto

Shinzo Kishimoto

Yoshikazu Amano

Nobuyoshi Sekikawa

Nobuo Hiratsuka

Isamu Hatanaka

Yutaka Akiyoshi

An integral multilayer analytical element for the analysis of an ammonia-forming substrate comprising:

Integral multilayer analytical element for analysis of ammonia-forming

A method of using a chemical analysis slide which includes a slide frame and a sheet-like or film-like chemical analysis element for colorimetric determination of a specific component in an aqueous liquid sample, which method includes

Method of using chemical analysis slide

A dipping element for immunoassay or quantitative analysis of an immunoreactive analyte is disclosed having a first matrix having a carrier therein containing an immobilized antibody capable of an immunochemical reaction with a sample antigen. The immobilized antibody is not released into the aqueous liquid sample upon contact. The element further comprises a second matrix which contains labelled antigen which does dissolve in the aqueous liquid sample upon contact. On dipping, the marker-labelled antigen reacts with the immobilized antibody in competition with the antigen-antibody reaction between the analyte antigen and the immobilized antibody. if the analyte is an antibody, then the second matrix contains a marker labelled antibody and the first matrix contains an immobilized antigen. Methods for the quantitative analysis of an analyte antigen or antibody are also disclosed.

Element for immunoassay and process of using the same

In an integral multilayer analytical element of a flat sheet type comprising a plurality of layers for quantitative analysis of an analyte in a liquid sample, the improvement which comprises at least two layers of a microporous sheet material arranged adjacent to each other, said at least two layers being combined by an adhesive provided in part on the interface of said layers, whereby the interface allows essentially uniform passage of a liquid therethrough.

Integral multilayer analytical element

A dry-type multilayer analytical element containing a color reagent composition comprising an oxidase specifically reaction with an analyte or derived therefrom to produce hydrogen peroxide, hydrogen peroxide-decomposing enzyme, an alkali metal salt of ferrocyanide and a chromogen which is separated from the hydrogen peroxide-decomposing enzyme, the chromogen being incorporated in a layer nearer to the transparent support of the analytical element than the layer containing the hydrogen peroxide-decomposing enzyme. The analytical element exhibits excellent coloration, color stability, and accuracy and the results are minimally influenced by the presence of hemoglobin in the sample.

Dry-type multilayer analytical element

A method of using a chemical analysis slide comprising a slide frame and a sheet-like or film-like chemical analysis element for colorimetric determination of a specific component in an aqueous liquid sample, which comprises

A method for quantitative determination of bilirubin is disclosed. The method comprises bringing a bilirubin-containing aqueous liquid sample into contact with a hydrophobic bilirubin extracting composition containing a hydrophobic amine capable of extracting bilirubin. The amine extracts the bilirubin in said aqueous liquid sample. Photometry is then used to determine the concentration of bilirubin extracted with the bilirubin extracting composition. A device for use in this method is also disclosed. The device has a layer containing a hydrophobic bilirubin extracting composition containing a hydrophobic amine capable of extracting bilirubin.

Multilayer device for detecting bilurubin

A dry type multilayer analysis element comprises at least one porous medium layer comprising a membrane filter, to which an antigen (or antibody) is immobilized, and at least one reagent layer through which a substance(s) which did not participate in an antigen-antibody reaction can permeate. The multilayer analysis element is effective for assaying components present in body fluids, blood, urine, etc., in a simple manner.

Multilayer analysis element

An integral flat container type analytical instrument for use in the quantitative analysis of an analyte present in a liquid sample which has a reaction chamber and an observation chamber associated with each other so as to allow a liquid to flow therebetween characterized in that: at least part of the wall of said reaction chamber is made of a flexible material; said observation chamber is provided with wall parts which are made of a transparent material and opposed to each other in such a manner that their relative positions are fixed to provide a given light path; and no partition wall preventing the free flow of the liquid substantially exists between said reaction chamber and said observation chamber. A method for optical measurement of an analyte in a liquid sample utilizing said analytical instrument is also disclosed.

Flat container type analytical instrument

Chemical analysis sheets comprising a reagent layer, a porous spreading layer and a cover layer provided with a small opening are disclosed. The cover layer prevents rapid evaporation of moisture to ensure a complete reaction between the chemical reagent and the ingredient tested for.

Multilayer chemical analysis member having an outer waterproof layer

Method of bilirubin detection

An integral multilayer analytical element for the analysis of ammonia or an ammonia forming substrate in a liquid sample is disclosed. The element comprises a transparent support on which an indicator layer for ammonia, a liquid blocking layer, a reagent layer incorporated with a reagent which may react with the substrate to form ammonia, and a spreading layer, are integrally superposed in this order. The improvement is that the liquid blocking layer is made of a porous material which is permeable to ammonia but substantially impermeable to liquids.

Integral multilayer analytical element for the analysis of ammonia or an

An analysis film for assaying transaminase activity comprises a reagent layer containing pyruvic acid oxidase, a combination of a hydrogen donor and a coupler--which is capable of forming a cationic dye--and an anionic polymer. The cationic dye formed by the reaction with a color indicator composition for detecting hydrogen peroxide is fixed with the anionic polymer, thereby mobilization and diffusion of the cationic dye being prevented so that the analysis film achieves high detection sensitivity and high accuracy.

Quantitative analysis film

A quantitative analysis film comprising a reagent layer comprising a cationic dye-forming color indicator for detecting hydrogen peroxide and an anionic polymer. By the use of such a color indicator and an anionic polymer in combination, a cationic dye is formed; the formed cationic dye is then bound and fixed to the anionic polymer to provide an intensified color. A method for the colorimetric assay of hydrogen peroxide or a precursor thereof with high sensitivity and high efficiency is also disclosed.

Quantitative analysis film and a method for colorimetric analysis using

A chemical analysis slide frame assembly including first and second slide frames each having an opening whose area is smaller than the area of a chemical analysis film inserted therein and a third slide frame disposed between the first and second slide frames. The third slide frame has an opening which is adapted to receive the chemical analysis film and is shaped so as to substantially prevent the displacement of the chemical analysis film. The third slide frame has a thickness which is equal to or larger than the thickness of the chemical analysis film. The side wall of the opening in the first slide frame has a small optical reflection factor, preferably is dark so as to provide a small optical reflection factor.

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Asaka, Japan

Asaji Kondo