Immunex Corporation

Amgen Inc.

Arvia Eleanor Morris

Rebecca E McCoy

Richard Jeffrey Armitage

Brian D Follstad

James N Thomas

William Christian Fanslow, Iii

Melanie K Spriggs

Jeffrey T McGrew

Pranhitha Reddy

Subhashini Srinivasan

Marylou G Gibson

Jian Wu

Shun Luo

Henry Lin

Jose Carlos Escobar

Matthew I Jerums

Amanda Kano

Aurora Villegas Viaje

Chi-Chang Lee

Erika Pineda

Leslie Phillip Miranda

Sohye Kang

Rohini Deshpande

Sharon Wong-Madden

John D Pluenneke

Rebecca E Mccoy

The invention provides a method for culturing mammalian cells. The method provides greater control over cell o growth to achieve high product titer cell cultures.

Mammalian cell culture

The invention provides a method for culturing mammalian cells. The method provides greater control over cell growth to achieve high product titer cell cultures.

The invention provides a method for culturing mammalian cells. The method provides greater control over cell growth to achieve high product titer cell cultures by changing the temperature of the cell culture and/or by starving the cells in their asparagine supply.

Methods for treating medical conditions induced by interleukin-4 involve administering an IL-4 antagonist to a patient afflicted with such a condition. Suitable IL-4 antagonists include, but are not limited to, IL-4 receptors (such as a soluble human IL-4 receptor), antibodies that bind IL-4, antibodies that bind IL-4R, IL-4 muteins that bind to IL-4R but do not induce a biological response, molecules that inhibit IL-4-induced signal transduction, and other compounds that inhibit a biological effect that results from the binding of IL-4 to a cell surface IL-4R. Particular antibodies provided herein include human monoclonal antibodies generated by procedures involving immunization of transgenic mice. Such human antibodies may be raised against human IL-4 receptor. Certain of the antibodies inhibit both IL-4-induced and IL-13-induced biological activities.

Anti-interleukin-4 receptor antibodies

The present invention relates to methods for mammalian cell culture. The methods make use of independent tyrosine and cystine feed streams.

Method for culturing mammalian cells to improve recombinant protein production

The invention provides stable feed media containing pyruvate and methods for stabilizing feed media by adding pyruvate. The invention further provides methods for producing proteins using such media and proteins produced through the use of such methods.

Feed media

The present invention relates to the culture of animal cells in serum-free culture medium. The present invention provides particular dipeptides that can improve recombinant protein production and cell viability in such cultures, especially in the absence of peptones.

Dipeptides to enhance yield and viability from cell cultures

The invention provides improved methods of recombinant protein production in cell culture. More specifically, the invention relates to the modulation of the IGF-1 signaling pathway in cells so as to improve production characteristics.

Methods and host cells for improved cell culture

The invention provides cells that produce increased levels of recombinant protein by modulating the activity of translational regulator gene products that are downstream targets of PKB alpha, methods of making such cells, and methods of using such cells. Such translational regulator gene products include 4E-BP1 and mTOR.

Growing community of inventors

Seattle, WA, United States of America

Arvia Eleanor Morris