Baxalta Gmbh

Baxter Healthcare Sa

Immuno Aktiengesellschaft

Baxter Aktiengesellschaft

Baxter International Inc.

Baxter Innovations Gmbh

Takeda Pharmaceutical Company Limited

Immuno Aktiengesellschaft Fur Chemisch-medizinische Produkte

Bio-products & Bio-engineering Ag

Bazalta Gmbh

Baxalta Innovations Gmbh

Artur Mitterer

Friedrich Dorner

Johann J Eibl

Manfred Reiter

Wolfgang Mundt

Bernhard Fischer

Meinhard Hasslacher

Christian Fiedler

Leopold Grillberger

Peter Turecek

Hans-Peter Schwarz

Uwe Schlokat

Christa Mayer

Christa Tauer

Falko-Guenter Falkner

Renate Den-Bouwmeester

Kathrin Thomas-Urban

Thomas R Kreil

Horst Schafhauser

&Ouml;yvind L Sch&ouml;nberger

Juergen Siekmann

Noel Barrett

Barbara Plaimauer

Falko-Gunter Falkner

Dominik Mittergradnegger

Simone Von Fircks

Michael Graninger

Heidi Meyer

Öyvind L Schönberger

Oyvind L Schönberger

Roland Geyer

James L McDonel

Thomas C Montie

Provided herein are methods for purifying recombinant A Disintegrin-like and Metallopeptidase with Thrombospondin Type 1 Motif 13 (ADAMTS13) protein from a sample. The method comprises enriching for ADAMTS13 protein by chromatographically contacting the sample with hydroxyapatite under conditions that allow ADAMTS13 protein to appear in the eluate or supernatant from the hydroxylapatite. The methods may further comprise tandem chromatography with a mixed mode cation exchange/hydrophobic interaction resin that binds ADAMTS13 protein. Additional optional steps involve ultrafiltration/diafiltration, anion exchange chromatography, cation exchange chromatography, and viral inactivation. Also provided herein are methods for inactivating virus contaminants in protein samples, where the protein is immobilized on a support. Also provided herein are compositions of ADAMTS13 prepared according to said methods.

Methods of purifying recombinant ADAMTS13 and other proteins and compositions thereof

The invention relates to a method for removing a viral contaminant from a preparation, being a cell culture medium or at least a component of a cell culture medium. The method comprises subjecting said preparation to filtration for at least about 24 hours through a virus filter having an effective pore size of maximum about 75 nm. Further, the invention relates to the use of a virus filter in filtration of at least about 24 hours, wherein the virus filter has an effective pore size of maximum about 75 nm for the removal of viral contaminant from a preparation, being a cell culture medium or at least a component of a cell culture medium. In some embodiments the filtration according to the invention operates at a volumetric capacity of at least about 2000 L/m. Further, the invention relates to the use of a preparation, being a cell culture medium or at least a component of a cell culture medium obtainable according to method of the invention for cell culture; pharmaceutical, diagnostic and/or cosmetic preparations as well as in food preparations.

Virus filtration of cell culture media

The present invention relates to a two-step method for the purification of divalent cation binding proteins with high yield and high purity on anion exchange resin materials, to divalent cation binding proteins obtainable by said method, and to a kit comprising means for carrying out said method.

Protein purification by anion exchange chromatography

The present invention relates to oligopeptide-free cell culture media comprising at least 0.5 mg/L of a polyamine and to methods for cultivating cells in said oligopeptide-free cell culture media comprising at least 0.5 mg/L of a polyamine. The invention also relates to methods for expressing at least one protein in a medium comprising at least 0.5 mg/L of a polyamine and to methods for producing at least one virus in a medium comprising at least 0.5 mg/L of a polyamine.

Oligopeptide-free cell culture media

The present invention relates to a method for the purification of divalent cation binding proteins with high purity on an anion exchange resin material, to divalent cation binding proteins obtainable by said method, and to a kit comprising means for carrying out said method.

Purification method for divalent cation binding proteins on anion exchange resin

The present invention relates to a method for the purification of Vitamin K dependent proteins with high yield and high purity, particularly enriched in active protein, on anion exchange resin materials, to Vitamin K dependent proteins obtainable by said method, and to a kit comprising means for carrying out said method.

Purification method for vitamin K dependent proteins by anion exchange chromatography

A medium is described for the protein-free and serum-free cultivation of cells, especially mammalian cells, whereby the medium contains a proportion of soy hydrolysate.

Medium for the protein-free and serum-free cultivation of cells

The present invention provides a method for purification of a virus or virus antigen comprising providing a virus preparation and centrifugation of said virus preparation in a gradient of a sugar established by the addition of two or more buffered sugar layers of different concentration. The method leads to higher yields and reduces unwanted aggregation of the virus or virus antigen by increasing the volume of the peak pool.

Formulation of sugar solutions for continuous ultracentrifugation for virus purification

The present invention provides methods for purifying Von Willebrand factor (VWF) for increased removal of non-lipid enveloped viruses.

Purification of VWF for increased removal of non-lipid enveloped viruses

Growing community of inventors

Orth/Donau, Austria

Artur Mitterer