Brandeis University

Arthur Reis

Lawrence J Wangh

John E Rice

J Aquiles Sanchez

Jesse J Salk

Kenneth E Pierce

Cristina Hartshorn

Aquiles J Sanchez

Kenneth Pierce

Provided herein are fluorescence detection methods for nucleic acid sequences and to kits for performing such methods.

Methods, kits and reaction mixtures for analyzing single-stranded nucleic acid sequences

Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled primers and probes, improves reproducibility and quantification. Low-temperature homogeneous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-PCR amplifications reduce complexity and permit 'single-tube' processing.

Primers, probes and methods for nucleic acid amplification

An additive for preventing mispriming in polymerase chain reaction (PCR) amplifications and assays comprising a hairpin oligonucleotide having a stem duplex greater than six nucleotides in length and a stabilized stem terminus. The additive improves PCR amplifications, including LATE-PCR amplifications when added to initial amplification reaction mixtures. It can be included in oligonucleotide sets and in kits for PCR amplification and assays.

Reagents and methods for improving reproducibility and reducing mispriming in PCR amplification

Growing community of inventors

Arlington, MA, United States of America

Arthur Reis