Biomerieux, Inc.

Biomerieux Sa

Idg (UK) Limited

Arthur James

Sylvain Orenga

John Perry

Stephen Stanforth

Vindhya Salwatura

Lyle Armstrong

Olivier Fabrega

Daniel Monget

Annette Rigby

Rosaleen Joy Anderson

Celine Roger-Dalbert

Paul William Groundwater

Andrey Victorovich Zaytsev

Vindhya Lakshika Salwatura

CÉLINE Roger-Dalbert

Céline Roger-Dalbert

The invention relates to the use of a compound having formula (I) as an enzymatic substrate for the detection of a nitroreductase activity, wherein: W, W, Wand Ware independently H, Br, Cl, F, I, alkyl, alkoxy, thiomethyl, perfluoroalkyl, nitro, cyano, carboxyl (including the esters or amides thereof) or any combination of same; n=0, 1 or 2; X is NR, CZZ, S or O, R being H, alkyl, aralkyl, aryl, alkanoic or alkylsulphonic, Zand Zbeing an alkyl; Y is N or NR, R being alkyl, aralkyl, aryl, alkanoic or alkylsulphonic; Z, Z, Zand Zare independently H, Br, Cl, F, I, alkyl, aryl, alkoxy, perfluoroalkyl, nitro, cyano, carboxyl, sulphonyl, including the sulphonyl or carboxyl amides or esters thereof, and the salts of same. 

Nitroreductase enzymatic substrates

The invention relates to the use of a compound having formula (I) as an enzymatic substrate for the detection of a nitroreductase activity, wherein: W, W, Wand Ware independently H, Br, Cl, F, I, alkyl, alkoxy, thiomethyl, perfluoroalkyl, nitro, cyano, carboxyl (including the esters or amides thereof) or any combination of same; n=0, 1 or 2; U is N or NR and V is CZN or NR or V is N or NR and U is CZ, R being H, alkyl, aralkyl, aryl, alkanoic or alkylsulphonic; and Z, Z, Z, Z, Zand Zare independently H, Br, Cl, F, I, alkyl, aryl, alkoxy, perfluoroalkyl, nitro, cyano, carboxyl, sulphonyl, including the sulphonyl or carboxyl amides or esters thereof, and the salts of same.

The present invention relates to the use of a compound of the following formula (I), as an enzyme substrate for the detection of a peptidase activity or as a pH indicator: according to which:

Peptidase substrates

The invention relates to the use of a compound having formula (I) as an enzymatic substrate for the detection of a nitroreductase activity or as an enzymatic substrate for the detection of a nitroreductase activity and indicator of pH, wherein: 

The present invention relates to the use of a compound of the following formula (I) for detecting a peptidase activity and/or a variation in pH: according to which:

The present invention relates to the use of a compound of the following formula (I) for detecting a peptidase activity and/or a variation in pH: 

The present invention relates to a method for identifying at least two groups of microorganisms expressing the same enzymatic activity, comprising the following steps:

Method for identifying at least two groups of microorganisms

An enzyme substrate of formula (I) detects peptidase activity: Methods of detecting peptidase activity of a microorganism may include incubating a biological sample with the enzyme substrate of formula (I), and detecting peptidase activity if a microorganism having peptidase activity is present in the biological sample. The enzyme substrate may be included in a reaction medium.

Substrates for detecting peptidase activity and methods using the substrates

The present invention relates to an enzyme substrate for detecting nitroreductase activity of formula (I) below: in which X is S, NX1, O or NX1-CO; R1 is nothing or a substituent selected from Cl, CH, Br, F, I, alkyl, aryl and carboxyl; R2 is nothing or a substituent selected from Cl, O&#x2014;CH&#x2014;O, O&#x2014;CH, F, diethylenediamine-CH, NR3R4, Br, I, alkyl, aryl, carboxyl, NOand R3 and R4 are independently H or an alkyl group containing from 1 to 4 carbon atoms; and X1 is selected from H, CH, CHPh, OH, alkyl and aryl.

Enzymatic phenoxazinone substrates of formula (I): in reaction media containing the same are used for detecting, identifying and/or quantifying microorganisms expressing at least one peptidase activity.

Enzymatic substrates derived from phenoxazinone and their use as developers in detection of microorganisms with peptidase activity

The present invention relates to methods for the detection, identification and/or quantification of microorganisms with peptidase activity using enzymatic substrates with the following general formula: 

Method for the detection, identification and/or quantification of microoganisms with peptidase activity using phenoxazinone derivatives as enzyme substrates

The invention relates to a medium for detecting and/or identifying microorganisms present in a sample, comprising a culture medium and at least one substrate that can be hydrolysed to a labelled product by at least a first enzyme not free in the sample, and specific for the microorganisms, characterized in that it also comprises at least one inhibitor of at least a second enzyme, different from the first enzyme or identical to it, but free in said sample and not originating from a microorganism. The present invention also finds a preferred application in the field of biomedical diagnosis or in food microbiology, and more particularly in bacteriology and mycology.

Medium for detecting and/or identifying of microorganisms

Novel enzymatic substrates of the general formula below: 

Enzymatic substrates derived from phenoxazinone and their use as developer in detection of microorganisms with peptidase activity

An alizarin-based chromogenic substrate, a composition containing the substrate, and its use to detect the presence of an enzymatic activity are disclosed. The substrate is particularly applicable in the field of biological diagnosis.

Alizarin-based chromogenic substrates, their uses and composition containing same

The present invention relates to novel enzymatic substrates with the following general formula: where R, R, R, R, R, R, A and X are as defined in claim 1, the reaction media comprising the same and the use thereof for the detection and/or identification and/or quantification of microorganisms expressing at least one peptidase activity.

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Cumbria, United Kingdom

Arthur James