Genentech, Inc.

Arthur D Levinson

Christian C Simonsen

Gordon A Vehar

David V Goeddel

William J Kohr

Diane Pennica

Elizabeth M Yelverton

Daniel G Yansura

William I Wood

Daniel J Capon

Richard M Lawn

Ronald A Hitzeman

Chung-Cheng Liu

Christian C Simonsen, Sr

A method for producing tissue plasminogen activator (t-PA) in eukaryotic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild-type DHFR with methotrexate.

Human tPA production using vectors coding for DHFR protein

A method for producing tissue plasminogen activator (t-PA) in eukaryotic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild type DHFR with methotrexate.

A method for producing tissue plasminogen activator (t-PA)in eukaroytic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild-type DHFR with methotrexate.

Human t-PA production using vectors coding for DHFR protein

Human t-PA production using vectors coding DHFR protein

A method for controlled production of a desired mature protein in a vertebrate host cell through the use of a polycistronic expression vector, which contains sequences coding for the desired protein and a secondary protein, is described. Both coding sequences are governed by the same promoter and are separated by translational stop and start signal codons. The expression of the secondary sequence effects control over the expression of the sequence coding for the desired protein, and the secondary protein functions as a marker for selection of transfected cells.

Polycistronic expression vector construction

A method for producing factor VIII in recombinant mammalian host cells utilizing an expression vector containing a selectable marker DNA and an amplifiable marker DNA. The initial selection is based upon the selectable marker and subsequent amplification of factor VIII DNA and amplifiable marker DNA is conducted in cells not deficient in the amplifiable marker.

Preparation of functional human factor VIII in mammalian cells using

Hepatitis surface antigen is synthesized in recombinant yeast hosts transformed with vectors encoding hepatitis surface antigen, preferably under the control of the yeast PGK promoter and preferably in the absence of DNA encoding the surface antigen precursor. Hepatitis surface antigen is assembled by yeast into antigenic 22 nm particles even though hepatitis surface antigen bacterial transformants were not known to be capable of assembling the surface antigen into 22 nm particles.

Preparation of hepatitis b surface antigen in yeast

Novel vaccines are provided for immunization against hepatitis B surface antigen wherein the surface antigen is present in 22nm form but contains only mature hepatitis B surface antigen.

Preparation of polypeptides in vertebrate cell culture

A polycistronic construction of xenogeneic sequences expressible in eukaryotic cells is described. DHFR encoding sequences under control of the same promoter with another foreign gene can be amplified with methotrexate, and thus cause coamplification of the foreign gene.

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Hillsborough, CA, United States of America

Arthur D Levinson