Illumina Corporation

Complete Genomics, Inc.

Ventana Medical Systems, Inc.

Arnold Oliphant

Mark S Chee

Jian-Bing Fan

Scott L Butler

John R Stuelpnagel

Min-Jui Richard Shen

Nils B Adey

Kenneth M Kuhn

Wanyuan Ao

Mun-Jui Richard Shen

John E Stuelpnagel

Scott Butler

A method for detecting nucleic acids by (a) providing a sample having target nucleic acids, each nucleic acid having contiguous first, second, and third domains; (b) contacting the sample with probe sets to form hybridization complexes, wherein each probe set includes (i) a first probe having a sequence that is complementary to the first domain; and (ii) a second probe having a sequence substantially complementary to the third domain; (c) extending the first probes along the second domains of the complexes while the complexes are immobilized on a solid support; (d) ligating the extended first probes to the second probes to form templates; (e) amplifying the templates with primers that are complementary to the first and second priming sequences to produce amplicons; and (f) detecting the amplicons on the surface of a nucleic acid array.

Multiplex nucleic acid reactions

A scalable reaction and detection system for automated high throughput sequencing of nucleic acids involving a combination of chemical processes and observation processes independent of the chemistry processes. Discrete functional units may be configured in a manner that allows the system to interchangeably utilize different sequencing reaction components in conjunction with discrete apparatus components for optical image collection and/or analysis.

Method for high throughput screening of nucleic acids

Apparatus for high throughput sequencing of nucleic acids

The invention is directed to a variety of multiplexing methods used to amplify and/or genotype a variety of samples simultaneously.

Growing community of inventors

Sunnyvale, CA, United States of America

Arnold Oliphant