Lanthiopep B.v.

Dsm IP Assets B.v.

Rijksuniversiteit Groningen

Arnold Jacob Mathieu Driessen

Johannes Andries Roubos

Cornelis Johannes Leenhouts

René Verwaal

Bianca Elisabeth Maria Gielesen

Bernard Meijrink

Rick Rink

Gert Nikolaas Moll

Oscar Paul Kuipers

Anneke Kuipers

Carsten Pohl

Roelof Arij Lans Bovenberg

Yvonne Irene NYGÅRD

The invention relates to the production of heterologous polypeptides in a recombinant host cell. More specifically, it relates to the production and secretion of peptides, such as biologically active peptides, that are modified by one or more lantibiotic-synthesizing enzymes. Provided is a nucleic acid construct encoding a polypeptide comprising 1) a non-lantibiotic export signal that is recognized by a non-lantibiotic export system; 2) a lantibiotic leader peptide that is recognized by at least a lantibiotic dehydratase such as LanB and, C-terminally of said export signal and said leader peptide, 3) a peptide of interest containing one or more serine or threonine residue(s) which can be posttranslationally dehydrated by said dehydratase. Also provided is a method for producing a polypeptide in a host cell, comprising providing a host cell with a nucleic acid construct according to the invention and allowing expression and secretion of the encoded polypeptide by said host cell, wherein said host cell comprises at least a dehydratase capable of modifying said encoded polypeptide and a non-lantibiotic export system capable of secreting said modified polypeptide.

Methods for the production and secretion of modified peptides

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Groningen, Netherlands

Arnold Jacob Mathieu Driessen