Pharmazell Gmbh

Arno Aigner

Dirk Weuster-Botz

Michael Braun

Werner Hummel

Rolf Schmid

Daniel Bakonyi

Boqiao Sun

Christina Kantzow

Sven Bresch

Luo Sha Liu

Lou Liu

Rolf D Schmid

Kyoko Momoi

Stefan Gaab

Alexander Hartung

Luo Liu

In various aspects and embodiments, the invention provides a nucleic acid molecule comprising a nucleotide sequence encoding a 7β-hydroxysteroid dehydrogenase (7β-HSDH) mutant that catalyzes at least the stereospecific enzymatic reduction of a 7-ketosteroid to the corresponding 7-hydroxysteroid, wherein the mutant has, compared to the wildtype 7β-HSDH of SEQ ID NO:2, a decreased substrate inhibition and/or an altered cofactor usage, and the mutant has, in comparison with the wildtype 7β-HSDH of SEQ ID NO:2, 1 to 15 amino acid additions, substitutions, deletions and/or inversions in the sequence motif VMVGRRE corresponding to positions 36 to 42 of SEQ ID NO:2.

7β-hydroxysteroid dehydrogenase mutants and process for the preparation of ursodeoxycholic acid

The invention relates to novel 7&#xdf;-hydroxysteroid dehydrogenase mutants, to the sequences which encode these enzyme mutants, to processes for the preparation of the enzyme mutants and to their use in enzymatic reactions of cholic acid compounds, in particular in the preparation of ursodeoxycholic acid (UDCS). The invention also relates to novel processes for the synthesis of UDCS using the enzyme mutants; and to the preparation of UDCS using recombinant, multiply-modified microorganisms.

7Beta-hydroxysteroid dehydrogenase mutants and process for the preparation of ursodeoxycholic acid

The invention relates to novel 7&#x3b2;-hydroxysteroid dehydrogenases which are obtainable from bacteria of the genus, especially of the strain, to the sequences encoding said enzymes, to methods for producing said enzymes and to their use in the enzymatic conversion of cholic acid compounds, and especially in the production of ursodeoxycholic acid (UDCS). The invention also relates to novel methods for the synthesis UDCS.

7&#x3b2;-hydroxysteroid dehydrogenases and their use

The invention relates to 12 &#x3b1;-hydroxysteroid dehydrogenases, nucleic acid sequences coding for the same, expression cassettes and vectors, recombinant microorganisms containing the corresponding coding nucleic acid sequences, a method for producing said 12 &#x3b1;-hydroxysteroid dehydrogenases, a method for enzymatic oxidation of 12 &#x3b1;-hydroxysteroids using said enzyme, a method for enzymatic reduction of 12-ketosteroids using said enzyme, a method for qualitative or quantitative determination of 12-ketosteroids and/or 12&#x3b1;-hydroxysteroids using said 12&#x3b1;-hydroxysteroid dehydrogenases and a method for production of ursodesoxycholic acid, comprising the enzyme-catalyzed cholic acid oxidation using said 12 &#x3b1;-hydroxysteroid dehydrogenases.

7 &#x3b1;-hydroxysteroid dehydrogenase knockout mutants and use therefor

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Tuntenhausen, Germany

Arno Aigner