Invitrogen Dynal As

Qiagen Gmbh

Qiagen As

Arne Deggerdal

Frank Larsen

Vidar Skagestad

Evy Reitan

The present invention provides a method of isolating nucleic acid from a sample, said method comprising contacting said sample with a detergent and a solid support, whereby soluble nucleic acid in said sample is bound to the support, and separating said support with bound nucleic acid from the sample. Where the method of the invention is used to isolate DNA, it may conveniently be couple with a further step to isolate RNA from the same sample.

Isolation of nucleic acid

A process for isolating nucleic acid from a nucleic acid-containing sample, which comprises: (a) providing a chaotrope; (b) providing a nucleic acid binding solid phase capable of binding nucleic acid in the presence of the chaotrope; (c) providing a source of NH4+ or NH3; (d) contacting the sample with the nucleic acid binding solid phase in the presence of a liquid phase comprising the chaotrope and the NH4+ or NH3; and (e) optionally separating the solid phase with the nucleic acid bound thereto from the liquid phase.

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Asker, Norway

Arne Deggerdal