Massachusetts Institute of Technology

The Broad Institute, Inc.

Arnaud Gutierrez

James Joseph Collins

Paul Blainey

Navpreet Ranu

Michael Andrew Lobritz

Sarah DiIorio

Joshua Elacqua

The present disclosure relates to compositions and methods for detection of single-stranded breaks (SSBs) in dsDNA, including in genomic DNA. Measurement of the precise location of SSB damage in DNA, e.g., genomic DNA, is provided, and involves an approach that is compatible with next-generation sequencing.

Single-stranded break detection in double-stranded DNA

Provided herein are pharmaceutical compositions and kits useful for sensitizing a microorganism or a population of microorganisms to a quinolone antibiotic. In a particular aspect, a carbon source and an electron acceptor can sensitize an antibiotic persistent microorganism to treatment with a fluoroquinolone antibiotic. Methods for sensitizing a microorganism to a quinolone antibiotic, reducing the density-dependent persistence (DDP) of an antibiotic resistant microorganism, and reducing the number of persistent cells in a population are also provided. Theses compositions and methods are useful in treating infections resulting from high-density bacterial cultures, such as pneumonia, genitourinary infections, biofilms, prosthetic graft infections, sepsis, and endovascular infections.

Sensitization of bacterial cells to quinolone antibiotics

Growing community of inventors

Somerville, MA, United States of America

Arnaud Gutierrez