Centre National De La Recherche Scientifique

Institut Curie

Sorbonne Université

Université Pierre Et Marie Curie

Paris Sciences Et Lettres

Paris Sciences Et Lettres-quartier Latin

Ecole Normale Superieure De Paris

Universite Pierre Et Marie Curie—paris 6 (Upmc)

Arnaud Gautier

Ludovic Jullien

Vincent Croquette

Franck Perez

Thomas Le Saux

Jérôme Querard

Pencheng Wang

Samantha Albright

Chenge Li

Nath Sarang

Fluorescent labeling of proteins. In particular, membrane-impermeant fluorogenic chromophores being capable of binding reversibly a functional derivative of a Photoactive Yellow Protein (PYP), or a functional fragment thereof, for fluorescently labeling biological molecules of interest, preferably proteins of interest. Especially, 4-hydroxybenzylidene-rhodanine (HBR) analogs of formula (II) as membrane-impermeant fluorogenic chromophores. 

Membrane-impermeant fluorogenic chromophores

A method for detection of at least one reversibly photo-convertible fluorescent species, comprises the following steps: a) illumination of a sample comprising said or at least one of the reversibly photo-convertible fluorescent species by a periodically modulated illuminating light; and b) detection of fluorescent emission emitted by the sample thus illuminated; wherein the method further comprises the following step: c) extraction of the amplitude of the intensity component of the fluorescent emission exhibiting the same periodicity as the periodically modulated illuminating light and a phase quadrature with respect to the same; and wherein the mean intensity of the illuminating light and the modulation frequency of the same are chosen to maximize the amplitude of the intensity component of the fluorescent emission.

Method for detection of a reversibly photo-convertible fluorescent species

Disclosed is a functional derivative of a Photoactive Yellow Protein (PYP), or a functional fragment thereof, for fluorescently labelling particles, e.g. proteins, or surfaces, which is capable of binding reversibly a fluorogenic chromophore of formula (I), and which is capable of enhancing the brightness of the fluorogenic chromophore upon complexation thereto; and of inducing the spectral shift of the fluorogenic chromophore through the ionization of an auxochromic group thereof.

Fluorogen activating and shifting tag (FAST)

Growing community of inventors

Paris, France

Arnaud Gautier