Biomerieux, Inc.

Commissariat a L'energie Atomique Et Aux Energies Alternatives

Commissariat a L'energie Atomique

Armelle Novelli-Rousseau

Francois Mallet

Mathieu Dupoy

Thierry Delair

Pierre Joly

Pierre Marcoux

Marie-Helene Charles

François Mallet

Florence Ricoul

Guy Oriol

Frédéric Mallard

Frédéric Ginot

Raphaël Mathey

Catherine Ladaviere

Valérie Cheynet

A method for observing a sample is provided. The method includes the steps of: a) providing a volume (G, V) of a substantially transparent medium having a first refractive index (n) and having a first surface (S) that is convex, rounded, or faceted, and a second substantially planar surface (S), the first surface separating the volume from a surrounding medium (MA) having a second refractive index (n) that is lower than the first index, the sample to be observed being contained inside the volume or being deposited on the first surface; b) observing the sample through the second substantially planar surface; and c) determining the presence of a substance or an object in the sample by detection during step b), a ring of light that correlates with the volume.

Method for observing a sample

Analysis chip of at least one analyte, said chip comprising at least one analysis spot for recognition and immobilization specific to the analyte; and a reference range (G) comprising several reference spots each arranged on said chip in a defined manner and independently of one another, each reference spot of this range comprising, immobilized on its surface and in a defined proportion P that is different and known for each spot relative to the other reference spots of said range: a probe reference molecule (PRM) permitting recognition and hybridization specifically to a defined target reference molecule (TRM), and/or an inert oligonucleotide molecule (IM) incapable of recognition and hybridization with said PRM, these molecules both being unable to immobilize said analyte or analytes. The method of the invention comprises the use of said chip with its reference range for analysis of said, at least one, analyte.

Analysis chip with reference range, kits and methods of analysis

The invention concerns a method for isolating a target biological material contained in a sample, which consists in providing a capture phase comprising an organic molecule having at least a reactive function and at least a protein material capable of recognizing or binding, specifically and directly or indirectly, with the target biological material, said protein material having a specific covalent binding site with the organic molecule reactive function, consisting of at least a tag comprising at least six contiguous lysine, or lysine derivative residues, the method consists in contacting said target biological material with at least the capture phase; and detecting the target biological material fixed on the capture phase: The invention also concerns the capture and detection phases, and a reagent containing them.

Method for isolating a target biological material, capture phase, detecting phase and reagent containing them

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Seyssins, France

Armelle Novelli-Rousseau