Cargill Inc.

Bioamber Inc.

Evolva, Inc.

Arlene M Fosmer

Brian Jeffrey Rush

Thomas W McMullin

Vernon L McIntosh, Jr

Ting Liu Carlson

Veronique Douchin

Gregory Michael Poynter

Christopher K Miller

Simon Carlsen

Kevin T Watts

James C Anderson

Beth M Mastel

Jeanette M Huryta

Hans Peter Smits

Kenneth R Finley

Jon Veldhouse

Keith M Brady

Peter Alan Jauert

Manuel Quiros Asensio

The present invention relates to genetically modified yeasts that can use lactate as a carbon source to produce a fermentation product. In one aspect, the yeasts can consume glucose and lactate simultaneously to produce ethanol. In one aspect, the genetically modified yeast is transformed to include a monocarboxylic/monocarboxylate transporter. In one aspect, the yeast can include one or more heterologous genes encoding lactate dehydrogenase (cytochrome) (EC 1.1.2.3 and/or 1.1.2.4).

Genetically modified lactate-consuming yeasts and fermentation processes using such genetically modified yeasts

Yeast cells having a reductive TCA pathway from pyruvate or phosphoenolpyruvate to succinate, and which include at least one exogenous gene overexpressing an enzyme in that pathway, further contain an exogenous transhydrogenase gene.

Yeast cells having reductive TCA pathway from pyruvate to succinate and overexpressing an exogenous NAD(P+) transhydrogenase enzyme

Disclosed are methods for producing steviol glycosides, such as rebaudioside D and rebaudioside M, using engineered yeast. The methods include at least two phases: first and second phases where a glucose-containing feed composition is provided to the medium in different modes of feeding in each phase, such as variable feeding and then constant feeding. The two phase feeding can result in a growth rate that is slower in the second phase than in the first phase, and consequently increased steviol glycoside production rates, reduced fermentation times, and reduced biomass concentrations.

Fermentation methods for producing steviol glycosides with multi-phase feeding

The present application provides genetically modified yeast cell comprising an active succinate fermentation pathway, as well as methods of using these cells to produce succinate.

Compositions and methods for succinate production

The present invention relates to genetically modified yeasts that can use lactate as a carbon source to produce a fermentation product. In one aspect, the yeasts can consume gluconse and lactate simultaneously to produce ethanol. In one aspect, the genetically modified yeast is transformed to include a monocarboxylic/monocarboxylate transporter. In one aspect, the yeast can include one or more heterologous genes encoding lactate dehydrogenase (cytochrome) (EC 1.1.2.3 and/or 1.1.2.4).

Yeast cells having reductive TCA pathway from pyruvate to succinate and overexpressing an exogenous NAD(P)+ transhydrogenase enzyme

Disclosed are methods for producing steviol glycosides, such as rebaudioside D and rebaudioside M, using engineered yeast. The methods include growing yeast on non-fermentative carbon sources. Other methods include growing yeast on one or more polysaccharides in which saccharification and fermentation of the polysaccharides occurs simultaneously.

Methods for producing steviol glycosides in engineered yeast

The present invention relates to a genetically engineered yeast capable of manufacturing a fermentation product using sucrose as a fermentation substrate, and fermentation processes using such a yeast. The yeast has an exogenous invertase gene and has a deletion or disruption of the PDC activity gene. Accordingly, the yeast is useful for manufacturing fermentation products other than ethanol from fermentation substrates containing sucrose.

Genetically modified yeasts and fermentation processes using genetically modified yeasts

Recombinant yeast cells contain a reductive TCA pathway from phosphoenolpyruvate or pyruvate to succinate. At least one metabolic step in the pathway includes a reaction of NADPH to produce NADP+. The yeast cell contains at least one exogenous NADPH-dependent gene in the pathway from phosphoenolpyruvate or pyruvate to succinate, preferably an NADPH-dependent malate dehydrogensase or fumarate reducase gene (or both).

Yeast cells having NADP(H)-dependent reductive TCA pathway from pyruvate to succinate

Provided herein are genetically modified yeast cells for the production of succinate, methods of making these yeast cells, and methods of using these cells to produce succinate.

Growing community of inventors

Eden Prairie, MN, United States of America

Arlene M Fosmer