Dyadic International (usa), Inc.

Dyadic International, Inc.

Other

Battelle Memorial Institute

Iowa Corn Promotion Board

Danisco US Inc.

Arkady Panteleimonovich Sinitsyn

Mark Aaron Emalfarb

Peter Jan Punt

Philip Terry Olson

Richard Paul Burlingame

Richard P Burlingame

Alexander Vasilievich Gusakov

Christine Marie Pynnonen

Martine Parriche

Jean Christophe Bousson

Arie Ben-Bassat

Boris B Ustinov

Oleg N Okunev

Marco A Baez

Cornelia Marie Johanna Van Zeijl

Tatyana N Salanovich

Alexey I Antonov

Elena Vlasenko

Jan Cornelis Verdoes

Zhijie Jiang

Vladimir Mikhaylovich Chernoglazov

Irina Vladimirovna Solovjeva

Mark Gosink

Sandra Wihelmina Agnes Hinz

Jon K Magnuson

Marion McKinley Bradford

Cornelia Maria Johanna Van Zeijl

Oleg Nicolaevich Okounev

Jacoba Van Der Meij

Mark Aaron Emalfrab

Oleg Nicolaevich Ocounev

Jacoba Van Der Meij, Legal Representative

Marco A Baez-Vasquez

Cornelia Maria Van Zeijl

This invention provides novel enzyme compositions using newly identified and isolatedenzymes, including CBH Ib CBH IIb, EG II, EG VI, &#x3b2;-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.

Construction of Highly efficient cellulase compositions for enzymatic hydrolysis of cellulose

A novel transformation system in the field of filamentous fungal hosts for expressing and secreting heterologous proteins or polypeptides is described. The invention also covers a process for producing large amounts of polypeptide or protein in an economical manner. The system comprises a transformed or transfected fungal strain of the genus, more particularly ofand mutants or derivatives thereof. It also covers transformants containingcoding sequences, as well expression-regulating sequences ofgenes. Also provided are novel fungal enzymes and their encoding sequences and expression-regulating sequences.

Transformation system in the field of filamentous fungal hosts

Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose

This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to a method to release cellular content by degradation of cell walls. The invention also relates to methods to use the novel enzymes and compositions of such enzymes in a variety of other processes, including washing of clothing, detergent processes, biorefining, deinking and biobleaching of paper and pulp, and treatment of waste streams.

BX11 enzymes having xylosidase activity

Methods to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material are provided, as well as novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass.

Methods and compositions for degradation of lignocellulosic material

This invention relates to enzymes and methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to methods to use the novel enzymes and compositions of such enzymes in a variety of other processes, including washing of clothing, detergent processes, deinking and biobleaching of paper and pulp, and treatment of waste streams.

Fungal enzymes

A computer-based method and apparatus for the analysis specification and support of work processes. The system is designed to support multiple interdependent decisions, at least some of which require collaboration among multiple participants (). Work processes are modeled using an application framework () used to develop abstract, decision () process models. The decision () process models are used as a pattern to instantiate concrete process models that incorporate the work defined by the abstract process. The process model is then used to instantiate project models that incorporate the required work from the process. The project models are used to direct and guide the behavior of the participants () in the work process.

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A novel transformation system in the field of filamentous fungal hosts for expressing and secreting heterologous proteins or polypeptides is described. The invention also covers a process for producing large amounts of polypeptide or protein in an economical manner. The system comprises a transformed or transfected fungal strain of the genus Chrysosporium, more particularly of and mutants or derivatives thereof. It also covers transformants containing Chrysosporium coding sequences, as well expression-regulating sequences of Chrysosporium genes. Also provided are novel fungal enzymes and their encoding sequences and expression-regulating sequences.

The subject invention relates to novel compositions of neutral and/or alkaline cellulase and methods for obtaining neutral and/or alkaline cellulase compositions from Chrysosporium cultures, in particular Chrysosporium lucknowense. This invention also provides mutants and methods of generating mutants of Chrysosporium capable of producing neutral and/or alkaline cellulase. This invention also relates to the genes encoding the enzymes comprising the neutral and/or alkaline cellulase composition. In addition, this invention provides methods of culturing Chrysosporium to produce neutral and/or alkaline cellulases. The neutral and/or alkaline cellulase compositions of the subject invention can be used in a variety of processes including stone washing of clothing, detergent processes, deinking and biobleaching of paper & pulp and treatment of waste streams.

Treating cellulosic materials with cellulases from chrysosporium

The subject invention relates to novel compositions of neutral and/or alkaline cellulase and methods for obtaining neutral and/or alkaline cellulase compositions from Chrysosporium cultures, in particular Chrysosporium lucknowense. This invention also provides mutants and methods of generating mutants of Chrysosporium capable of producing neutral and/or alkaline cellulose. This invention also relates to the genes encoding the enzymes comprising the neutral and/or alkaline cellulase composition. In addition, this invention provides methods of culturing Chrysosporium to produce neutral and/or alkaline cellulases. The neutral and/or alkaline cellulase compositions of the subject invention can be used in a variety of processes including stone washing of clothing, detergent processes, deinking and biobleaching of paper & pulp and treatment of waste streams.

Cellulase compositions and methods of use

Growing community of inventors

Moscow, Russia

Arkady Panteleimonovich Sinitsyn