Plant Bioscience Limited

Institute of Food Research

Arjan Narbad

Michael John Gasson

Nicholas John Walton

Michael John Charles Rhodes

Melinda Mayer

The present invention relates to novel polypeptides derived from endolysins from a bacteriophage ofand to nucleic acid molecules encoding the same, as well as compositions thereof. The invention also provides uses of such polypeptides and nucleic acid molecules in the diagnosis and treatment of conditions and diseases associated with microbial cells such as. In particular, the invention provides a polypeptide having endolysin activity derived from bacteriophage &#x3a6;CP51 ofand uses thereof.

Polypeptides having endolysin activity and uses thereof

A method of producing vanillin comprising the steps of: (1) providing trans-ferulic acid or a salt thereof; and (2) providing trans-ferulate: CoASH ligase activity (enzyme activity I), trans-feruloyl ScoA hydratase activity (enzyme activity II), and 4-hydroxy-3-methoxyphenyl-&bgr;-hydroxy-propionyl SCoA (HMPHP SCoA) cleavage activity (enzyme activity III). Conveniently the enzymes are provided by Fe3 or a mutant or derivative thereof. Polypeptides with enzymes activities II and III and polynucleotides encoding the polypeptides. Use of the polypeptides or the polynucleotides in a method for producing vanillin is also provided.

Production of vanillin

A method of producing vanillin comprising the steps of: (1) providing trans-ferulic acid or salt thereof; and (2) providing trans-ferulate; CoASH ligase activity (enzyme activity I) trans-feruloyl SCoA hydratase activity (enzyme activity II), and 4-hydroxy-3-methoxyphenyl-&bgr;-hydroxy-propionyl SCoA (HMPHP SCoA) cleavage activity (enzyme activity III). Conveniently the enzymes are provided by,Fe3 or a mutant or derivative thereof. Polypeptides with enzymes activities II and III and polynucleotides encoding said polypeptides. Use of said polypeptides or said polynucleotides in a method for producing vanillin.

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Norwich Norfolk, United Kingdom

Arjan Narbad