Gevo, Inc.

Cargill Inc.

Valtion Teknillinen Tutkimuskeskus

Natureworks LLC

Danisco Sweeteners Oy

California Institute of Technology

Aristos Aristidou

Catherine Asleson Dundon

Andrew Hawkins

Pirkko Suominen

Peter Meinhold

Kari Koivuranta

Laura Ruohonen

Jun Urano

Patrick R Gruber

Brian Jeffrey Rush

Marja Ilmen

William Andrew Evanko

David A Glassner

Doug Lies

Matthew W Peters

Vineet Rajgarhia

Christopher K Miller

Stacey Olson

Thomas Buelter

Pim Van Hoek

Aharon Eyal

Merja Penttilä

Uvini Gunawardena

Reid M Renny Feldman

Kent Evans

Julie Kelly

Fudu Miao

Stephanie Porter-Scheinman

Merja Elisa Penttila

Benjamin Matthew Hause

Christopher Smith

Kevin Roberg-Perez

Justas Jancauskas

Ruth Berry

Lynne Albert

Melissa Dey

Holly J Jessen

Hans H Liao

Ana Negrete-Raymond

John Londesborough

Thomas W McMullin

James Wade

Peter Richard

Mervi Toivari

Jian Yi

Joshua Lundorff

Marco Landwehr

Frances H Arnold

Hans Söderlund

Heikki Ojamo

Chris Miller

Hakan Gros

Sabine Bastian

Josef Deutscher

Merja Pentilla

Andrei Miasnikov

Anita Teleman

Pirrko Suominen

Matthew Miller

Mira Povelainen

Claire Plazanet-Menut

Thomas William McMULLIN

Merja Penttilae

Matthew Peters

Merja Pentillä

Eva Eckl

Renny Feldman

Reid M Feldman

Yeast cells are genetically modified to disrupt a native metabolic pathway from dihydroxyacetone to glycerol. In certain aspects, the yeast cell is of the generaor. In other aspects, the yeast cell is capable of producing at least one organic acid, such as lactate. The yeast cells produce significantly less glycerol than the wild-type strains, and usually produce greater yields of desired fermentation products. Yeast cells of the invention often grow well when cultivated, despite their curtailed glycerol production.

Yeast cells having disrupted pathway from dihydroxyacetone phosphate to glycerol

Fermentation process using yeast cells having disrupted pathway from dihydroxyacetone phosphate to glycerol

The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

Yeast organism producing isobutanol at a high yield

The present application discloses the identification of the novelxylose transporter genes KHT105 and RAG4, as well as the identification of a novel set ofpentose phosphate pathway genes The present application further discloses a series of genetically modified yeast cells comprising various combinations of arabinose fermentation pathways, xylose fermentation pathways, pentose phosphate pathways, and/or xylose transporter genes, and methods of culturing these cells to produce ethanol in fermentation media containing xylose.

Compositions and methods for increased ethanol production from biomass

Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications include deletion of non-specific or specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.

Genetically modified yeast species, and fermentation processes using genetically modified yeast

The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of theGlade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

Yeast microorganisms with reduced by-product accumulation for improved production of fuels, chemicals, and amino acids

Specific oxygen uptake (OUR) is used as a process control parameter in fermentation processes. OUR is determined during at least the production phase of a fermentation process, and process parameters are adjusted to maintain the OUR within desired ranges. The invention is particularly applicable when the fermentation is conducted using a microorganism having a natural PDC pathway that has been disrupted so that it no longer functions. Microorganisms of this sort often produce poorly under strictly anaerobic conditions. Microaeration controlled by monitoring OUR allows the performance of the microorganism to be optimized.

Fermentation process using specific oxygen uptake rates as a process control

Methods for producing a biofuel are provided. Also provided are biocatalysts that convert a feedstock to a biofuel.

Methods for the economical production of biofuel from biomass

This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allows recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs.

Recovery of higher alcohols from dilute aqueous solutions

The present invention provides recombinant mircoorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of theclade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

Growing community of inventors

Highlands Ranch, CO, United States of America

Aristos Aristidou