Synthetic Genomics, Inc.

Viridos, Inc.

Exxonmobile Research and Engineering Company

Ariel S Schwartz

Robert Christopher Brown

Leah Soriaga

Rekha Seshadri

Soyan Leung Lieberman

Robert D Nielsen

Shaun Bailey

Jay McCarren

Jonathan E Meuser

Joseph C Weissman

Daniel Yee

Anna E Romano

Robert C Brown

Jay Mccarren

Roger C Prince

The present invention relates to the culture and manipulation of microorganisms for biotech applications, and is based on the discovery and characterization of spliced leader sequences identified in transcripts fromspecies. In particular, the invention provides nucleic acid compositions comprising a SL sequence operably linked to a protein-encoding gene. Further provided are compositions and methods for enhanced gene expression in recombinant microorganisms as well as methods for identification and/or isolation of nucleic acid molecules tagged with a spliced leader sequence.

spliced leader sequences and methods of use thereof

Mutant photosynthetic microorganisms having reduced chlorophyll and increased photosynthetic efficiency are provided. The mutants have a locked in high light-acclimated phenotype, in which many of the photosynthetic parameters characteristic of high light acclimated wild type cells are found in the LIHLA mutants when acclimated to low light, such as reduced chlorophyll, reduced NPQ, higher qP, higher E, higher Pper unit chlorophyll with little to no reduction in Pper cell, and higher rates of electron transport through photosystem II over a wide range of light intensities. Provided herein are constructs for attenuating or disrupting genes are provided for generating mutants having the LIHLA phenotype. Also provided are methods of culturing LIHLA mutants for the production of biomass or other products.

Algal mutants having a locked-in high light acclimated phenotype

Nannochloropsis spliced leader sequences and uses therefor

Growing community of inventors

San Diego, CA, United States of America

Ariel S Schwartz