Environmental Diagnostics, Inc.

Other

Immutron, Inc.

Arden A Kelton

Michael L Bell

Roy A Chung

Siong I Wie

William P Waters

David G Shrunk

A rotary fluid manipulator utilizable as a diagnostic device includes a porous body having fluid passages defined therein by fluid blocking means in the porosities of the body such as openings or slots in the body or compaction of areas of the porous body to define fluid passages therebetween. Various substances or binding partners such as receptors, immunoassay or other assay test materials and test specimens can be deposited on the porous body to permit various types of tests to be made by rotation of the manipulator to cause conjunctive centrifugal and wicking induced flow of fluids deposited thereupon.

Rotary fluid manipulator

A test card incorporates a colorimetric indicator test to determine the presence of a minute amount of a specific substance in a liquid medium. The test card includes top and bottom sheets adhesively secured to an intermediate frame member which, together with the top and bottom sheets, defines a filter chamber having a flat filter therein which incorporates a test portion. The test portion of the filter has a binding substrate to which antibodies of the specific substance have been bound. This binding substrate is in communication with a test port. In the method of the invention a substance to be tested is administered through the test port and contains an unknown amount of antigen. After the sample is administered an aqueous solution of enzyme labelled antigen is administered and, subsequently, a liquid substrate is added to cause a color change in the filter below the test port inversely proportional to the amount of antigen contained in the sample administered at the test port.

Test kit for determining the presence of organic materials and method of

A rotary fluid manipulator utilizable as a diagnostic device includes a porous body having fluid passages defined therein by fluid blocking means in the porosities of the body such as openings or slots in the body or compaction of areas of the porous body to define fluid passages therebetween. Various substances or binding partners such as receptors, immunoassay or other assay test materials and test specimens can be deposited on the porous body to permit various types of test to be made by rotation of the manipulator to cause conjunctive centrifugal and wicking induced flow of fluids deposited thereupon.

A test card incorporates a colorimetric indicator test to determine the presence of a minute amount of a specific substance in a liquid medium. The test card includes top and bottom sheets adhesively secured to an intermediate frame member which, together with the top and bottom sheets, defines a filter chamber having a flat filter therein which incorporates a test portion. The test portion of the filter has a binding substrate to which antibodies of the specific substance have been bound. This binding substrate is in communication with a test port. In the method of the invention a substance to be tested is administered through the test port and contains an unknown amount of antigen. After the sample is administered an aqueous solution of enzyme labelled antigen is administered and, subsequently, a liquid substrate is added to cause a color change in the filter below the test portion inversely proportional to the amount of antigen contained in the sample administered at the test port.

A liquid suspension separator of simple, one-piece construction is provided for field tests such as bovine testing. The liquid suspension separator includes a housing having a separator chamber incorporating a filter body characterized by its ability to laterally filter the particulates of the suspension from the liquid component thereof. The housing incorporates eluant, liquid suspension and discharge ports and the liquid component of the suspension can be immediately collected to permit a test or tests to be run thereupon. One of the embodiments of the separator includes a test chamber which already incorporates a test specimen reactive to the liquid component of the suspension to permit an immediate test to be conducted in the field.

Suspension liquid separator

A stabilized radioimmunoassay product consisting of an antibody protein-bound to the cell wall of a selected bacterium whereby the antibody is irreversibly bound to the protein and remains specific for the antigen against which it was developed. The radioimmunoassay product is also characterized by the fact that the resultant complete product includes a phase of serum protein treatment to isolate the protein sites or other sites of nonspecific reactivity with the labelled antigen such that the non-specific binding of labelled antigen is significantly reduced. Also within the contemplation of the invention is the method of manufacturing the radioimmunoassay product which includes the initial phase of protein binding of the antibody to the selected bacterium and the subsequent serum treatment to isolate previously unutilized protein sites such that when said sites are subsequently exposed to labelled antigen it will be rejected. Further within the contemplation of the invention is the method of utilizing the aforesaid product to totally eliminate the necessity for the customary incubation stages necessitated by prior art radioimmunoassay techniques.

Rapid radioimmunoassay product and method of making and using same

A rotatable element for use in centrifugal chemical-medical analysis apparatus wherein inlet means is provided in the element near the center of rotation thereof and an outlet is provided near the marginal radially outward edge thereof. A measuring chamber is provided intermediate the inlet and outlet and a conduit is afforded to such chamber from the inlet. A passageway is provided in the element extending radially inwardly from the chamber to a predetermined point or position relative to such chamber, and then to an overflow outlet to thereby define a predetermined filling level for the chamber. Fluid entering the inlet of the rotating element flows simultaneously into the chamber and the passageway under the centrifugal force, the fluid at said predetermined point relative to said measuring chamber overflowing to thereby define the filling level in the chamber.

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Westminster, CA, United States of America

Arden A Kelton