University of Central Florida Research Foundation, Inc.

University of Texas System

University of Massachusetts

Ardalan Naseri

Shaojie Zhang

Thoru Pederson

Hanhui Ma

Ahsan Sanaullah

Degui Zhi

Erwin Holzhauser

Scot Andrew Wolfe

Maximilaan Huisman

Metewo Selase Kosi Enuameh

Nicola Anne Kearns

Rene Maehr

Manuel Garber

Li-Chun Tu

Ryan Michael Jude Genga

A system for indexing, updating, and search haplotypes for genetic genealogical discovery in genotype databases. The system includes a pool of genetic indexes, a haplotype ingestion engine, and a haplotype query engine. The haplotypes of a number of individuals in a genotype database are indexed by a pool of multiple panels, and each panel pool can be dynamically updated by the insertion or deletion of individual haplotypes. A genetic genealogical search of a query haplotype against the database is achieved by first projecting the query onto a subset of panels in the pool, then conducting long match queries over each panel, and finally aggregating the identified long matches into Identical-by-Descent segments, i.e., DNA matches, between the query and the haplotypes in the database.

Methods and system for efficient indexing for genetic genealogical discovery in large genotype databases

The present disclosure relates to methods of and systems for modifying the transcriptional regulation of stem or progenitor cells to promote their differentiation or reprogramming of somatic cells. Further, the labeling and editing of human genomic loci in live cells with three orthogonal CRISPR/Cas9 components allow multicolor detection of genomic loci with high spatial resolution, which provides an avenue for barcoding elements of the human genome in the living state.

Compositions and methods for differential Cas9 gene labeling and/or editing

A C9orf72 DNA repeat expansion can be detected using a CRISPR Arrayed Repeat Detection System (CARDS). Based upon the compositions and methods supporting this platform primary cell cultures and/or blood cell smears can be tested under conventional clinical diagnostic laboratory conditions to diagnose genetically-based diseases having DNA repeat expansions, including but not limited to ALS. dCas9 constructs are also contemplated as having fluorescent proteins bound to any or all stem loop sequences, wherein detection of a plurality of dCas9 constructs having different colored fluorescent proteins can simultaneously detect at least six (6) different gene target loci.

Detection of gene loci with CRISPR arrayed repeats and/or polychromatic single guide ribonucleic acids

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Orlando, FL, United States of America

Ardalan Naseri