The General Hosptial Corporation

Arda Mizrak

Xandra O Breakefield

Mehmet Fatih Bolukbasi

Okay Saydam

Disclosed herein is an isolated nucleic acid molecule comprising a first nucleic acid sequence 5'-ACCCTGCCGCCTGGACTCCGCCTGT-3&#x2032; (SEQ ID NO: 22), or a functional variant thereof, operably linked to a second, heterologous nucleic acid sequence. The isolated nucleic acid molecule can be DNA (in an expression vector) and RNA (mRNA, shRNA, orncRNA). Also disclosed is a microvesicle comprising the nucleic acid molecule and a microvesicle preparation comprising the microvesicle. Also disclosed is an in vitro method of producing a microvesicle preparation enriched for a specific RNA sequence by transfecting cells with the nucleic acid sequence, and isolating microvesicles generated therefrom. Methods of delivering therapeutic RNA to a subject are also disclosed.

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San Francisco, CA, United States of America

Arda Mizrak