Corning Incorporated

Aravind Raghavan Rammohan

Nikolaos Pantelis Kladias

Shawn Michael O'Malley

Po Ki Yuen

Joseph Christopher Wall

David A Kenney

Yulong Hong

James M Philippe

Paul Kevin Seeto

Neng Wang

Kathy Marie Youngbear

Sumitava De

Jin Wang

Shang-Pin Kwei

Michael Patrick Keenan

David Alan Kenney

A closure assembly is provided. The closure assembly includes a cap portion, a tubular neck extending from a top surface of the cap portion, and at least one reinforcing member extending between the top surface of the cap portion and an exterior surface of the tubular neck, wherein the cap portion, the tubular neck and the at least one reinforcing member are a unitary integral piece. The closure assembly design provides stability to the tubular neck and the cap portion when a force is applied to either one or both of the tubular neck and the cap portion.

Reinforced barbed closure

A perfusion bioreactor and a method for using the perfusion bioreactor for performing a continuous cell culture are disclosed. The perfusion bioreactor includes an outer vessel having a housing with a gas permeable membrane, an opening and a cavity; an inner vessel within the cavity; and a lid to cover the opening. A porous membrane within the cavity divides the cavity into inner and outer compartments. A fresh media port extends through the outer vessel or the at least one lid to receive a fresh media tube that has an end located in the inner compartment. A spent media port extends through the outer vessel or the at least one lid to receive a spent media tube that has an end located in the outer compartment. A mixer is within the inner compartment, and the porous membrane is attached to an opening within the inner vessel.

Perfusion bioreactor and method for using same to perform a continuous cell culture

A 5-liter bioreactor vessel enables a substantial increase in the volume of biological media that can be cultivated in the vessel compared to conventional designs. Moreover, when agitated at 1.5&#xd7; the shaking frequency of a conventional 3-liter bioreactor (i.e., 90 rpm versus 60 rpm), the 5-liter vessel achieves a 19% increase in cell aeration without exceeding the maximum shear stress limit for cell viability. The 5-liter vessel optionally includes a plurality of internal baffles configured to disrupt the liquid vortex and reduce the maximum shear stress transferred to biomedia contained within the vessel.

Vessel for growth of biological entities

Growing community of inventors

Big Flats, NY, United States of America

Aravind Raghavan Rammohan