Emory University

Rutgers, The State University of New Jersey

Arash Grakoui

Joseph Marcotrigiano

Timothy R Sampson

David S Weiss

Aryn Alaine Price

Jillian L Whidby

The present disclosure relates to constructs useful in expressing biotinylated monomers and tetramers produced from these monomers. The present disclosure also relates to methods for production and use of these tetramers in identifying and isolating antigen specific B cells and cloning antibodies thereto.

Compositions and methods for identifying and sorting antigen-specific B cells

This disclosure relates to Cas9-nucleic acid complexes and uses related thereto. In certain embodiments, the disclosure contemplates transgenic plants and animals genetically engineered to express Cas9-nucleic acid complexes disclosed herein. In certain embodiments, the disclosure relates to methods of treating or preventing, diseases, conditions, cancer, viral infections or other pathogenic infection using vectors configured to express a Cas9-nucleic acid complex disclosed herein.

CAS9-nucleic acid complexes and uses related thereto

The present disclosure relates to constructs useful in expressing biotinylated monomers and tetramers produced from these monomers. Specifically, the disclosure provides a construct useful in expressing biotinylated antigen monomers, said construct comprising a viral protein of an ectodomain of Hepatitis C Virus (HCV) envelope glycoprotein E2 or Human Immunodeficiency Virus (HIV) gp140. Further disclosed are methods for production and use of these tetramers in identifying and isolating antigen specific B cells and cloning antibodies thereto.

Cas9-nucleic acid complexes and uses related thereto

Methods of producing and using Hepatitis C virus (HCV) eE2 polypeptides are described.

HCV E2 construct compositions and methods

This disclosure relates to viral particles and nucleic acids encoding an HCV envelope glycoprotein 2 containing a mutation. Viral particles can be created and administered to a subject to illicit an immune response.

Growing community of inventors

Decatur, GA, United States of America

Arash Grakoui