Ciba-Geigy Corporation

Novartis Ag

Aran Paulus

Carlo S Effenhauser

Andreas Helg

Peter Oroszlan

Carlo Stefan Effenhauser

Kees Ensing

Gerardus Jacobus Bruin

An improved microstructure for micro-scale fluid handling, processing and investigation comprises a support element and a microstructured element. The support element has a substantially even support face. The microstructured element has a microstructured face provided both with substantially even portions and with recessed portions. The even portions are substantially coplanar to a common even surface. The recessed portions are defined between the even portions adjacent thereto and recessed from the common even surface. The support face is located substantially adjacent to the microstructured face and cooperates with the recessed portions thereof to define a microchannel system. At least the microstructured element is made and formed of poly(dimethylsiloxane) polymer by applying a prepolymer or a precursor thereof onto a corresponding master element, and then curing the material.

Microstructure device

A device for combined bioaffinity assay and electrophoretic separation is provided, which comprises a capillary system having two stages, a first stage in which bioaffinity interactions of analyte molecules and molecular recognition elements are performed, and a second stage, in which electrophoretic separation of the analyte molecules and subsequent detection of the separated species is accomplished. Within the first capillary stage the molecular recognition elements are attached and immobilized to the inside capillary wall, for example, by adsorption or by covalent binding to the capillary material. The method for combined bioaffinity assay and electrophoretic separation comprises flowing an analyte through a capillary system having two stages. In a first capillary stage the analyte molecules are captured by respective molecular recognition elements present in that stage. More particularly the analyte molecules are captured by molecular recognition elements which are attached and immobilized to the inside wall of that capillary stage, for example, by adsorption or by covalent binding to the capillary material. After a predetermined time the analyte-molecules are dissociated from the molecular recognition elements. Subsequently the analyte-molecules are separated in a second stage of the capillary system by capillary electrophoresis and finally the separated species are detected at the terminal part of the capillary system.

Device and method for combined bioaffinity assay and electrophoretic

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Heitersheim, Germany

Aran Paulus