Mgi Tech Co., Ltd.

Bgi Shenzhen

Complete Genomics, Inc.

Qingdao Mgi Tech Co., Ltd

Egi Tech (shen Zhen) Co., Limited

Egi Tech Co, Limited

Ao Chen

Wenwei Zhang

Xun Xu

Chongjun Xu

Erkai Liu

Jin Yang

Hui Xing Wang

Lin He

Sha Liao

Jing Zhao

Yuan Jiang

Bin Xie

Xia Zhao

Hang Zhang

Ming Ni

Wen-wei Zhang

Hanjie Shen

Junqiang Xu

Hui Wang

Shengming Zhao

Zhuokun Li

Defeng Fu

Jie Zhao

Hengzhi Wang

Provided are a rolling circle amplification method, a method for preparing a sequencing library, and a DNA nanoball prepared therefrom. The rolling circle amplification method includes: sequentially denaturing and annealing a double-stranded DNA and a mediating sequence in a same system, to complementarily pair the mediating sequence with two ends of a denatured single-stranded DNA; simultaneously introducing a ligase and a polymerase into the system to connect the two ends of the single-stranded DNA under action of the ligase; and performing a rolling circle amplification reaction under action of the polymerase by using the mediating sequence as a primer and the single-stranded DNA as a template, to obtain DNA nanoball.

Rolling circle amplification method, method for preparing sequencing library, and DNA nanosphere prepared therefrom

Provided is a method for sequencing a long-fragment nucleic acid. The nucleic acid molecules each containing a long insert, a first sequencing adapter, and a second sequencing adapter, is used to construct a sequencing library, and the sequencing is performed in segments to sequence the nucleic acids having the long inserts.

Method for sequencing long-fragment nucleic acid

The present invention provides a method for sequencing polynucleotides. Sequential incorporation of different nucleotides is detected by using the same light emitting signal, thereby achieving the determination of polynucleotide sequences.

Method for sequencing polynucleotides

A separation and collection device for cells and biomolecules includes an annular tube and several magnetic beads configured to identify and bind with different cells or molecules in the biological sample. The annular tube defines a through hole for the biological sample to exit. Sizes of different groups of magnetic beads are different. An aperture size of a through hole is changeable such that the plurality of groups of magnetic beads can flow through the through hole in a certain order. A testing system including the separation and collection device is further disclosed.

Separation and collection device for cells and biomolecules, and testing system

A nucleic acid probe and a nucleic acid sequencing method for performing sequencing while ligating nucleic acids. The nucleic acid probe is a DNA sequencing probe, comprising a first moiety, a second moiety, a linker, and a detectable label. A base of the first moiety is A, T, U, C, or G, a base of the second moiety is a random base and/or a universal base, and 3 bases or more are present in the second moiety. The first moiety and the second moiety are ligated via the linker, the connection between the first moiety and the ligation can be cleaved, and the detectable label is ligated to the second moiety or the linker. The above probe, a combination formed therewith, or a sequencing method using the same can reduce the number or types of probes in nucleic acid sequencing, thereby reducing cost.

Nucleic acid probe and nucleic acid sequencing method

The present invention provides a method for sequencing a nucleic acid using an immersion reaction protocol. The immersion reaction protocol comprises sequentially immersing a solid support having nucleic acid molecules immobilized thereon in different reaction containers to realize nucleic acid sequencing.

Nucleic acid sequencing method

Provided are a nucleic acid sequencing method and a nucleic acid sequencing kit. The kit comprises a nucleic acid probe, a ligase, dNTP having a blocking group attached to a 3' end, a polymerase, a reagent 1 for excising the blocking group attached to the 3&#x2032; end of the dNTP, and a reagent 2 for excising the remaining nucleotides on the nucleic acid probe that are not bound to a to-be-tested base group.

Nucleic acid sequencing method and nucleic acid sequencing kit

The present invention provides a method for improving the loading of nucleic acid on a solid support by contacting the solid support with a poloxamer-containing reagent. The present invention also provides a method for improving the stability of a nucleic acid on a solid support, comprising contacting a nucleic acid molecule with a partially double-strand oligonucleotide before or after loading the nucleic acid molecule on a solid support, so as to cause the nucleic acid molecule to hybridize with the oligonucleotide. The present invention also provides a combined use of the two methods.

Method for improving loading and stability of nucleic acid

Provided is a single fluorescent dye based sequencing method. Moreover, the present invention further provide modified nucleosides and nucleotides, and a kit comprising the nucleoside and/or nucleotide, particularly suitable for the sequencing method of the present invention. Additionally, the present invention further provides uses of the nucleoside, the nucleotide and the kit for sequencing.

Single fluorescent dye-based sequencing method

Growing community of inventors

Shenzhen, China

Ao Chen