General Electric Company

Ge Healthcare Bio-sciences Corp.

Amersham Biosciences Corp

Other

Leica Microsystems Cms Gmbh

Global Life Sciences Solutions USA LLC

Ce Healthcare Bio-sciences Corp.

Centre for Probe Development and Commercialization

Anup Sood

John Richard Nelson

Shiv R Kumar

Michael John Gerdes

Arunkumar Natarajan

Carl Williams Fuller

Lakshmi Sireesha Kaanumalle

Robert John Filkins

Michael Christopher Montalto

Fiona Ginty

Alberto Santamaria-Pang

Evelina Roxana Loghin

Qing Li

Ernest William Kovacs

Zhengyu Pang

Kwok Pong Chan

Reginald Donovan Smith

Wei Cai

Michael James Rishel

Su Lu

James Edward Rothman

John Yupeng Gui

Gregory Andrew Grossmann

Kevin Bernard Kenny

Vandana Keskar

Dmitry Vladimirovich Dylov

Tao Wu

Bruce Fletcher Johnson

Peter Miller

John Frederick Graf

Mark McDougall

Ali Can

Hongyi Zhou

Wei Gao

Kashan Ali Shaikh

Brion Daryl Sarachan

Randall Lee Carter

John Fitzmaurice Valliant

Brian David Polizzotti

Liming Yu

Nichole Lea Wood

Sean Richard Dinn

Yousef Ahmed Al-Kofahi

Christopher James Sevinsky

Maximilian Lewis Seel

Christina Lowes

Bahram Moasser

Zhida Pan

Zhixin Zheng

Hae Won Lim

John J Macklin

Liangliang Qiang

Lisa Anne Lowery

John Nelson

John Donald Burczak

Praveena Mohan

Elizabeth Mary McDonough

Thomas Pirrie Treynor

Chrystal Mae Chadwick

Martin James Brown

Michael Ernest Marino

Vidya Pundalik Kamath

John E Macklin

Anuradha Sekher

Paul Mitsis

Daniel Eugene Meyer

Aaron Joseph Dulgar-Tulloch

Karin Ann Stephenson

Yang Yang

Raghav Krishna Padmanabhan

Yunxia Sui

Sudhakar Rao

Melinda Larsen

Maureen Ann Bresnahan

Nicole Evelyn LaPlante

Mark Felipe Baptista

Su Lv

Padmaparna Chaudhuri

Padmaparna Chadhuri

Antti Eljas Seppo

Elizabeth Mary Collins

John Burczak

Lisa Lowery

Fiona Mary Ginty

Elizabeth Mary Mcdonough

Provided herein are methods of activating immune cells. The method includes providing a population of immune cells and contacting the population of immune cells with a first agent and a second agent. The first agent includes an immune cell activator attached to a first binder moiety, and the second agent includes at least one capture oligomer. The at least one capture oligomer is capable of associating with the first binder moiety. Also provided are kits for activating immune cells.

Methods and kits for cell activation

The invention discloses a method for reduction of autofluorescence in biological samples, comprising the steps of:

Method for reduction of autofluorescence from biological samples

The present invention relates to a microbubble complex comprising a microbubble having an outer shell comprising a mixture of native and denatured albumin encapsulating a perfluorocarbon gas, a therapeutic agent, a bifunctional linker having one end attached to the therapeutic agent and the other attached to a ligand and wherein the ligand is bound to the other shell of the microbubble through hydrophobic interactions. Also included are methods for delivering the aforementioned microbubble complex to a tissue target.

Microbubble complexes and methods of use

Methods comprising the use of photoactivated chemical bleaching for detecting multiple targets in a biological sample are provided. The methods include the steps of binding at least one probe to one or more target present in a biological sample including multiple targets, and detecting a signal from the probe. The method further includes the steps of contacting the sample comprising the bound probe with a photoinitiator and irradiating the sample, thereby initiating a photoactivated chemical bleaching process that substantially inactivates the probe. The method further includes the steps of binding at least one probe to one or more target present in the sample, and detecting a signal from the probe. The process of binding, detecting and bleaching may be iteratively repeated.

Chemical bleaching of dyes using radical photoinitiators

The subject matter of the present disclosure generally relates to techniques for image analysis. In certain embodiments, various morphological or intensity-based features as well as different thresholding approaches may be used to segment the subpopulation of interest and classify object in the images.

Systems and methods for automatic generation of training sets for machine interpretation of images

Provided are methods and kits for activating T cells, the method comprising providing a population of T cells, adding a plurality of first agents, where the first agent comprises a T-cell activator and a first binder moiety, and adding a second agent comprising a plurality of capture oligomers, where at least a segment of at least one of the plurality of capture oligomers is capable of associating with the first binder moiety. The method further comprises incubating the population of T cells, whereby at least a portion of the population of T cells is activated.

Methods comprising the use of photoactivated chemical bleaching for detecting multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample including multiple targets, binding at least one probe to one or more target present in the sample, and detecting a signal from the probe. The method further includes the steps of contacting the sample comprising the bound probe with an electron transfer reagent, as well as an optional additive which prevents target modification during photoactivated chemical bleaching, and irradiating the sample, thereby initiating a photoreaction that substantially inactivates the probe by photoactivated chemical bleaching. The method further includes the steps of binding at least one probe to one or more target present in the sample, and detecting a signal from the probe. The process of binding, defecting and bleaching may be iteratively repeated.

Photoactivated chemical bleaching of dyes

Methods comprising the use of photoactivated chemical bleaching for detecting multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing multiple targets, binding at least one probe to one or more target present in the sample, and observing a signal from the probe. The method further includes the steps of contacting the sample comprising the bound probe with a cationic or zwitterionic borate compound and irradiating the sample, thereby initiating a photoreaction that substantially inactivates the probe by photoactivated chemical bleaching. The method further includes the steps of binding at least one probe to one or more target present in the sample, and observing a signal from the probe. The process of binding, observing and bleaching may be iteratively repeated.

Photoactivated chemical bleaching of dyes using borates

Disclosed are novel computer-implemented methods for creating a blood vessel map of a biological tissue. The methods comprise the steps of, accessing image data corresponding to multi-channel multiplexed image of a fluorescently stained biological tissue manifesting expression levels of a primary marker and at least one auxiliary marker of blood vasculature, and extracting features of blood vessels using the primary marker as an input to create a single channel segmentation of the blood vessels. The method further comprises the steps of extracting features of blood vessels using the auxiliary marker to create auxiliary channels as a second input and apply multi-channel blood vessel enhancement. Blood vessel maps are created using the features and tracing the blood vasculature by iteratively extending the centerlines of the initial segmentation using statistical models and geometric rules.

System and method for blood vessel analysis and quantification in highly multiplexed fluorescence imaging

The disclosed embodiments are directed to a method for accurately counting and characterizing multiple cell phenotypes and sub-phenotypes within cell populations simultaneously by exploiting biomarker co-expression levels within cells of different phenotypes in the same tissue sample. The disclosed embodiments are also directed to a simple intuitive interface enabling medical staff (e.g., pathologists, biologists) to annotate and evaluate different cell phenotypes used in the algorithm and the presented through the interface.

Method and system for classification and quantitative analysis of cell types in microscopy images

Methods comprising probing multiple targets in an H&E stained biological sample are provided. The methods include the steps of providing a hematoxylin and eosin stained biological sample containing multiple targets, optionally detecting H&E staining of the sample, removing the hematoxylin and eosin signals, and detecting additional features or targets in the biological sample. The detecting step may include performing the steps of binding at least one probe to one or more targets to the sample, detecting a signal from the probe and contacting the sample with a bleaching agent. The process of binding, detecting and bleaching may be iteratively repeated.

Methods of analyzing an H and E stained biological sample

A borate compound for use as photoinduced chemical bleaching reagent is disclosed having improved bleaching performance under certain conditions, compared to neutral borates. The compounds are comprised of Formula I;RRRRBX&#x2003;&#x2003; Iwhere each R, R, and Rand Ris, independently, an alkyl, alkylaryl, or arylalkyl, with the proviso that at least one of R, R, and Rand Ris an alkyl group and at least two of R, R, Rand Rgroup is positively charged; X is an anion; and a equals 1, 2, or 3.

Borates for photoactivated chemical bleaching

Methods for detecting a plurality of targets in a biological sample are provided. The method comprises contacting the biological sample with a plurality of target-binding probes to form a plurality of target-bound probes, covalently attaching at least one of the target-bound probes to the biological sample, and observing the signals from the target-bound probes sequentially. An associated kit and device for detection of the plurality of targets are also provided.

Detection of plurality of targets in biological samples

Dynamic linking of pathway maps and cell maps is disclosed in certain embodiments. In such embodiments, the pathway maps are linked to spatially-localized regional nucleic acid data (e.g., sequence data), as opposed to non-spatially selected nucleic acid data. The pathway map and cell map data may be linked so that interactions results in changes or updates to the linked map, such as the selection or highlighting of cells exhibiting pathway map characteristics specified by a user of updating of node values or states to correspond to that of a cell or cells selected by the user.

Biological data annotation and visualization

Growing community of inventors

Clifton Park, NY, United States of America

Anup Sood