Dsm IP Assets B.v.

Technische Universiteit Delft

Danisco US Inc.

Antonius Jeroen Adriaan Van Maris

Jacobus Thomas Pronk

Victor Gabriel Guadalupe Medina

Ioannis Papapetridis

Hendrik Wouter Wisselink

Maarten Dirk Verhoeven

Paul Klaassen

Paulus Petrus De Waal

Jozef Petrus Johannes Schmitz

Niels Lauret

Rene Marcel De Jong

Johannes Pieter Van Dijken

Aaron Adriaan Winkler

Johannes Hendrik Winde

Johannes Hendrik De Winde

Jacobus Thomas Jan Pronk

The invention relates to a recombinant yeast cell, in particular a transgenic yeast cell, functionally expressing one or more recombinant, in particular heterologous, nucleic acid sequences encoding ribulose-1,5-biphosphate carboxylase oxygenase (Rubisco) and phosphoribulokinase (PRK). The invention further relates to the use of carbon dioxide as an electron acceptor in a recombinant chemotrophic micro-organism, in particular a eukaryotic micro-organism.

Recombinant yeast expressing rubisco and phosphoribulokinase

The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.

Fermentative glycerol-free ethanol production

The invention relates to a variant of a parent polypeptide which is preferably a hexose transporter, wherein the variant comprises an amino acid sequence which, when aligned with the amino acid sequence of SEQ ID NO: 1, comprises a substitution of amino acids N376 and T89, the positions of said amino acids being defined with reference to the amino acid sequence of SEQ ID NO: 1. Said polypeptide is suitable in the production of ethanol from arabinose-rich biomass such as corn stover and corn starch.

Polypeptides with improved arabinose transport specificity

The invention relates to a method for preparing a yeast which is capable of simultaneously fermenting a pentose and a hexose sugar, the method comprising providing a yeast which comprises: one or more heterologous genes encoding an enzyme of a pentose metabolic pathway, a disruption of a gene encoding a ribulose-phosphate 3-epimerase and a disruption of a gene encoding a glucose-6-phosphate isomerase, and one or more overexpressed endogenous genes encoding an enzyme of the pentose phosphate pathway; and subjecting the yeast to evolutionary engineering on a medium comprising a hexose sugar and at least one pentose sugar, selecting for a yeast with improved growth rate when grown on a media comprising a hexose and at least one pentose sugar, so as to obtain an evolved yeast.

Yeast cell capable of simultaneously fermenting hexose and pentose sugars

The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the 'PRK promoter') that enables higher expression under anaerobic conditions than under aerobic conditions.

Recombinant yeast cell

The present invention relates to a eukaryotic cell that is genetically modified comprising one or more heterologous gene encoding:

Eukaryotic cell with increased production of fermentation product

This invention relates to a recombinant cell, preferably a recombinant yeast cell comprising: a) a gene coding for an enzyme having glycerol-3-phosphate dehydrogenase activity, wherein said enzyme has a cofactor dependency for at least NADPand/or for NADPH; b) a gene encoding an enzyme having at least NADdependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10); and c) a mutation or disruption in at least one gene selected from the group of GPD1 and GPD2. Said cell is suitable for ethanol production, has a reduced glycerol production at high ethanol yield.

Growing community of inventors

Delft, Netherlands

Antonius Jeroen Adriaan Van Maris