University System of Maryland

Canon U.s.a., Inc.

Canon U.S. Life Sciences, Inc.

Antonio Fiore

Giuliano Scarcelli

Jitao Zhang

Hanyoup Kim

A multi-stage parallel spectroscopy system has a plurality of dispersion stages, with the output of one dispersion stage serving as input to the next dispersion stage. Each dispersion stage separates input radiation into respective spectral components along a respective dispersion axis. In embodiments, the dispersion axes for the dispersion stages are substantially parallel to each other. Thus, the disclosed systems may be considered single-axis parallel spectroscopy configurations, in contrast to cross-axis parallel spectroscopy configurations. An optical system disposed in an optical path between dispersion stages can spatially filter a set of wavelengths from the input to the next dispersion stage to increase spectral extinction without sacrificing throughput or parallel operation. In some embodiments, the same dispersive element provides the spectral separation for multiple dispersion stages, by way of a recirculating optical system that redirects the spectral output from the dispersive element back to its input.

Multi-stage parallel spectroscopy systems and methods

The present invention relates to a method and system for a label-free cell analysis based on Brillouin light scattering techniques. Combined with microfluidic technologies according to the present invention, Brillouin spectroscopy constitutes a powerful tool to analyze physical properties of cells in a contactless non-disturbing manner. Specifically, subcellular mechanical information can be obtained by analyzing the Brillouin spectrum of a cell. Furthermore, a novel configuration of Brillouin spectroscopy is provided to enable simultaneous analysis of multiple points in a cell sample.

Growing community of inventors

Hyattsville, MD, United States of America

Antonio Fiore