Chinoin Gyogyszer Es Vegyeszeti Termekek Gyara Rt.

Richter Gedeon Vegyeszeti Gyar Rt

Biogal Gyogyszergyar

Antonia Jekkel Nee Bokany

Imre Moravcsik

Gabor Ambrus

Karoly Albrecht

Sandor Piukovich

Miklos Jarai

Istvan Gado

Sandor Istvan

Kalman Konczol

Istvan M Szabo

Attila Andor

Tibor Lang

Janos Erdei

Valeria Szell

Eva Ilkoy

Gyorgy Santha

Eva Toth-Sarudy

Kalman Polya

Bela Makadi

Emilia Simonovits

Eva Tomori Nee Joszt

Lajos Kiss

Karoly Nagy

Gabor Hantos

Gyorgy Szvoboda

Zsuzesanna Vida

Gy Uml/o/ Szvoboda

Istvan Mihaly

Agota Hulber Nee Dobos

Bela Palotas

Karoly Buzasi

Etelka Deli Nee Konszky

Aniko Molnar Nee Antal

Zsuzsanna Lengyel Nee Szemenyei

Vilma Szaszhegyesi

Eva Csajagi

The invention relates to a microbial process for the production of immunosuppressive cyclosporine antibiotic complex or of its components, cyclosporine A, cyclosporine B and cyclosporine C, by the aerobic fermentation of a filamentous fungus strain biosynthesizing the above antibiotic(s) in a nutrient medium containing utilizable carbon and nitrogen sources as well as mineral salts, and by isolating the products formed, which comprises culturing a strain of the novel Tolypocladium varium fungus species producing the cyclosporine antibiotic complex, preferably tolypocladium varium sp. nov. CY/93, deposited at the National Collection of Agricultural and Industrial Microorganisms, Budapest, Hungary under the number NCAIM(P)F 001005, on a nutrient medium containing carbon sources, organic and inorganic nitrogen sources as well as mineral salts, under aerobic conditions, at 25.degree. to 30.degree. C., and, if desired, isolating and purifying the cyclosporine antibiotic complex or its components produced.

Microbial process for the production of immunosuppressive antibiotics

The invention relates to a process for the production of 9.alpha.-hydroxy-4-androstene-3,17-dione from natural sterols of plant or animal origin or from mixtures thereof by the submerged aerobic fermentation of an enzyme-deficient, sterol-de-grading microorganism in a nutrient medium containing utilizable carbon and nitrogen sources as well as mineral salts, and by isolating the product formed, which comprises transforming the sterol or sterol mixture with the culture of a 1,2-steroid-dehydrogenase deficient strain of new Mycobacterium roseum species which is able to degrade the side-chain of natural sterols, preferably with Mycobacterium roseum sp. nov. 1108/1 deposited at the National Collection of Agricultural and Industrial Microorganisms, Budapest, Hungary under the number NCAIM B (P) 000339, and by isolating and purifying the 9.alpha.-hydroxy-4-androstene-3,17-dione obtained.

Microbiological process for preparing

A process of producing sisomycin by cultivating a new species of Micromonospora.

Method for the preparation of antibiotic sisomicin

A culture of Micromonospora rosea capable of producing the antibiotic sisomicin.

Micromonospora culture

Growing community of inventors

Budapest, Hungary

Antonia Jekkel Nee Bokany