Biogemma UK Limited

Gene Shears Pty. Limited

Nickerson Biocem Limited

Antoni Ryszard Slabas

Adrian Paul Brown

Emma Jane Wallington

Jeroen Alexander Wilmer

Clare Louise Brough

Johannes Theodorus Maria Kroon

The invention provides a method of producing, in a plant, oil having an erucic acid content above 66% erucic acid, the method comprising (i) expressing in the plant nucleic acid encoding an elongase and nucleic acid encoding an acyltransferase enzyme; and (ii) extracting oil from the plant. Corresponding plants seeds and oils are also provided.

Oil biosynthesis

Plants, particularly transgenic plants, may be produced having a 2-acyltransferase enzyme from Limnanthes with an altered substrate specificity compared to the native enzyme. For example, oil seed rape ( ) may contain the 2-acyltransferase transgene derived from in order to produce trierucin. The cDNA sequence of 2-acyltransferase and its equivalents protein sequence are disclosed.

DNA sequence encoding plant 2-acyltransferase

Plants, particularly transgenic plants, maybe produced having a 2-acyltransferase enzyme or other insoluble acyltransferase enzyme with an altered substrate specificity compared to the native enzyme. For example, oil seed rape (,) may contain a 2-acyltransferase transgene derived from,in order to increase the erucic acid content of the oil. The cDNA sequence of maize (,) 2-acyltransferase is disclosed and is useful for cloning acyltransferase genes and/or CDNAs from other organisms, including

Plant expressing 2-acyltransferase

Plants, particularly transgenic plants, may be produced having a 2-acyltransferase enzyme or other insoluble acyltransferase enzyme with an altered substrate specificity compared to the native enzyme. For example, oil seed rape (Brassica napus) may contain a 2-acyltransferase transgene derived from Limnanthes douglassi in order to increase the erucic acid content of the oil. The cDNA sequence of maize (Zea mays) 2-acyltransferase is disclosed and is useful for cloning acyltransferase genes and/or cDNAs from other organisms, including L. douglassi.

Growing community of inventors

High Shincliffe, United Kingdom

Antoni Ryszard Slabas