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Biotecnol S.a.

Anton Zavialov

Timo Korpela

Vladimir Zav'yalov

Natalia Battchikova

Sheila Macintyre-Ayane

Lada Petrovskaya

Galina Petrovna Korobko

The present invention provides antimicrobials for efficient therapy of infections caused by bacterial pathogens. The antimicrobials include peptides corresponding to the active site of N-terminal extension of subunits composing specific adhesive organelles of virulence of pathogenic bacteria. These antimicrobial peptides inhibit surface expression of adhesive organelles and thus prevent development of infections caused by pathogenic bacteria. The invention describes also effective methods for screening molecules inhibiting the generation of the adhesive organelles.

Antimicrobials against pathogenic bacteria and method for screening them

The present invention provides bacterial strains for secretion of soluble biologically active recombinant heterologous proteins into periplasm or on a surface/into a cultivation medium of bacteria. The invention exploits the secretion system of Gram-negative bacteria including periplasmic chaperones and usher/secretin proteins. For accomplishing the aim, the bacterial strains simultaneously express the fusion protein (signal peptide)-(mature hecterologous protein)-(subunit of a bacterial surface structure, Caf1), periplasmic chaperone specific for the subunit, and outer membrane usher/secretin protein specific for the subunit. Secretion of fusion proteins: (signal peptide of Caf1)-(mature human IL-1&#x3b2;)-(mature Caf1), (signal peptide of Caf1)-(mature human GM-CSF)-(mature Caf1), and (signal peptide of Caf1)-(mature human IL-1ra)-(mature Caf1) that were expressed insimultaneously with the periplasmic chaperone Caf1M and the usher/secretin protein Caf1A are examples of the use of the invention.

Microbial protein expression system

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Moscow, Russia

Anton Zavialov